Abstract
Pineal graft from young to old rats was performed and red blood cell hemolysis, induced by the water-soluble radical initiator 2,2′-azobis (2-amidino-propane) dihydrochloride (AAPH), was evaluated 6 months after graft. Pineal graft modified the hemolysis curve kinetic profile: in grafted rats versus age-matched controls, the 50% hemolysis time as well as the lag time were longer, whereas the maximal amount of hemolysis was lower, and it occurred over a longer period of time. Thiobarbituric acid reactive substances production was lower in pineal-grafted rats than in controls and the age-related decrease of erythrocyte membrane fluidity was prevented by pineal graft. The present findings support an important physiological role of pineal gland in preventing age-related alterations of erythrocyte membranes and suggest a possible antioxidant action of melatonin.
| Original language | English |
|---|---|
| Pages (from-to) | 339-344 |
| Number of pages | 6 |
| Journal | Biogerontology |
| Volume | 5 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2004 |
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Keywords
- Aging
- Hemolysis
- Melatonin
- Peroxyl radicals
- Pineal graft
- TBARS
ASJC Scopus subject areas
- Geriatrics and Gerontology
Cite this
Pineal graft in old rats improves erythrocyte resistance to peroxyl radical-induced hemolysis. / Moroni, Fausto; Marcheselli, Fiorella; Recchioni, Rina; Fattoretti, Patrizia; Bertoni-Freddari, Carlo.
In: Biogerontology, Vol. 5, No. 5, 2004, p. 339-344.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Pineal graft in old rats improves erythrocyte resistance to peroxyl radical-induced hemolysis
AU - Moroni, Fausto
AU - Marcheselli, Fiorella
AU - Recchioni, Rina
AU - Fattoretti, Patrizia
AU - Bertoni-Freddari, Carlo
PY - 2004
Y1 - 2004
N2 - Pineal graft from young to old rats was performed and red blood cell hemolysis, induced by the water-soluble radical initiator 2,2′-azobis (2-amidino-propane) dihydrochloride (AAPH), was evaluated 6 months after graft. Pineal graft modified the hemolysis curve kinetic profile: in grafted rats versus age-matched controls, the 50% hemolysis time as well as the lag time were longer, whereas the maximal amount of hemolysis was lower, and it occurred over a longer period of time. Thiobarbituric acid reactive substances production was lower in pineal-grafted rats than in controls and the age-related decrease of erythrocyte membrane fluidity was prevented by pineal graft. The present findings support an important physiological role of pineal gland in preventing age-related alterations of erythrocyte membranes and suggest a possible antioxidant action of melatonin.
AB - Pineal graft from young to old rats was performed and red blood cell hemolysis, induced by the water-soluble radical initiator 2,2′-azobis (2-amidino-propane) dihydrochloride (AAPH), was evaluated 6 months after graft. Pineal graft modified the hemolysis curve kinetic profile: in grafted rats versus age-matched controls, the 50% hemolysis time as well as the lag time were longer, whereas the maximal amount of hemolysis was lower, and it occurred over a longer period of time. Thiobarbituric acid reactive substances production was lower in pineal-grafted rats than in controls and the age-related decrease of erythrocyte membrane fluidity was prevented by pineal graft. The present findings support an important physiological role of pineal gland in preventing age-related alterations of erythrocyte membranes and suggest a possible antioxidant action of melatonin.
KW - Aging
KW - Hemolysis
KW - Melatonin
KW - Peroxyl radicals
KW - Pineal graft
KW - TBARS
UR - http://www.scopus.com/inward/record.url?scp=21644449819&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=21644449819&partnerID=8YFLogxK
U2 - 10.1007/s10522-004-2572-1
DO - 10.1007/s10522-004-2572-1
M3 - Article
C2 - 15547321
AN - SCOPUS:21644449819
VL - 5
SP - 339
EP - 344
JO - Biogerontology
JF - Biogerontology
SN - 1389-5729
IS - 5
ER -