Pirin delocalization in melanoma progression identified by high content immuno-detection based approaches

Silvia Licciulli, Chiara Luise, Andrea Zanardi, Luca Giorgetti, Giuseppe Viale, Luisa Lanfrancone, Roberta Carbone, Myriam Alcalay

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Pirin (PIR) is a highly conserved nuclear protein originally isolated as an interactor of NFI/CTF1 transcription/replication factor. It is a member of the functionally diverse cupin superfamily and its activity has been linked to different biological and molecular processes, such as regulation of transcription, apoptosis, stress response and enzymatic processes. Although its precise role in these functions has not yet been defined, PIR expression is known to be deregulated in several human malignancies.Results: We performed immunohistochemical analysis of PIR expression in primary samples from normal human tissues and tumors and identified a dislocation of PIR to the cytoplasm in a subset of melanomas, and a positive correlation between cytoplasmic PIR levels and melanoma progression. PIR localization was subsequently analyzed in vitro in melanoma cell lines through a high content immunofluorescence based approach (ImmunoCell-Array).Conclusions: The high consistency between in vivo and in vitro results obtained by immunohistochemistry and ImmunoCell-Array provides a validation of the potential of ImmunoCell-Array technology for the rapid screening of putative biological markers, and suggests that cytoplasmic localization of PIR may represent a characteristic of melanoma progression.

Original languageEnglish
Article number5
JournalBMC Cell Biology
Volume11
DOIs
Publication statusPublished - Jan 20 2010

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Melanoma
Biological Phenomena
Nuclear Proteins
Fluorescent Antibody Technique
Neoplasms
Cytoplasm
Transcription Factors
Biomarkers
Immunohistochemistry
Apoptosis
Technology
Cell Line
In Vitro Techniques

ASJC Scopus subject areas

  • Cell Biology

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Pirin delocalization in melanoma progression identified by high content immuno-detection based approaches. / Licciulli, Silvia; Luise, Chiara; Zanardi, Andrea; Giorgetti, Luca; Viale, Giuseppe; Lanfrancone, Luisa; Carbone, Roberta; Alcalay, Myriam.

In: BMC Cell Biology, Vol. 11, 5, 20.01.2010.

Research output: Contribution to journalArticle

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abstract = "Background: Pirin (PIR) is a highly conserved nuclear protein originally isolated as an interactor of NFI/CTF1 transcription/replication factor. It is a member of the functionally diverse cupin superfamily and its activity has been linked to different biological and molecular processes, such as regulation of transcription, apoptosis, stress response and enzymatic processes. Although its precise role in these functions has not yet been defined, PIR expression is known to be deregulated in several human malignancies.Results: We performed immunohistochemical analysis of PIR expression in primary samples from normal human tissues and tumors and identified a dislocation of PIR to the cytoplasm in a subset of melanomas, and a positive correlation between cytoplasmic PIR levels and melanoma progression. PIR localization was subsequently analyzed in vitro in melanoma cell lines through a high content immunofluorescence based approach (ImmunoCell-Array).Conclusions: The high consistency between in vivo and in vitro results obtained by immunohistochemistry and ImmunoCell-Array provides a validation of the potential of ImmunoCell-Array technology for the rapid screening of putative biological markers, and suggests that cytoplasmic localization of PIR may represent a characteristic of melanoma progression.",
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AU - Licciulli, Silvia

AU - Luise, Chiara

AU - Zanardi, Andrea

AU - Giorgetti, Luca

AU - Viale, Giuseppe

AU - Lanfrancone, Luisa

AU - Carbone, Roberta

AU - Alcalay, Myriam

PY - 2010/1/20

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N2 - Background: Pirin (PIR) is a highly conserved nuclear protein originally isolated as an interactor of NFI/CTF1 transcription/replication factor. It is a member of the functionally diverse cupin superfamily and its activity has been linked to different biological and molecular processes, such as regulation of transcription, apoptosis, stress response and enzymatic processes. Although its precise role in these functions has not yet been defined, PIR expression is known to be deregulated in several human malignancies.Results: We performed immunohistochemical analysis of PIR expression in primary samples from normal human tissues and tumors and identified a dislocation of PIR to the cytoplasm in a subset of melanomas, and a positive correlation between cytoplasmic PIR levels and melanoma progression. PIR localization was subsequently analyzed in vitro in melanoma cell lines through a high content immunofluorescence based approach (ImmunoCell-Array).Conclusions: The high consistency between in vivo and in vitro results obtained by immunohistochemistry and ImmunoCell-Array provides a validation of the potential of ImmunoCell-Array technology for the rapid screening of putative biological markers, and suggests that cytoplasmic localization of PIR may represent a characteristic of melanoma progression.

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