Pituitary adenylate cyclase-activating polypeptide (PACAP) stimulates adenylate cyclase and promotes proliferation of mouse primordial germ cells

Maurizio Pesce, Rita Canipari, Gian Luca Ferri, Gregorio Siracusa, Massimo De Felici

Research output: Contribution to journalArticle

Abstract

During migration and for about 2 days after their arrival in the gonadal ridges, primordial germ cells (the embryonic precursors of gametes of the adult animal) proliferate actively. Certain growth factors, such as stem cell factor and leukemia inhibitory factor, seem to be essential for survival, proliferation and possibly differentiation of mouse primordial germ cell in vivo and/or in vitro. Similarly, increase in intracellular cAMP is followed by a marked enhancement of primordial germ cell proliferation, at least in culture. In the present study, we show that pituitary adenylate cyclase-activating polypeptides (PACAP-27 and PACAP-38), two neuropeptides of the secretin-glucagon-vasoactive intestinal polypeptide-GH-releasing hormone family, stimulate in vitro proliferation of mouse primordial germ cells, bind to primordial germ cells and gonadal somatic cells (possibly to type I PACAP receptor) and activate adenylate cyclase in the same cells. Moreover, PACAP-like immunoreactivity was found in gonadal ridges, mostly on germ cell surface. In conclusion, evidence is provided that PGC proliferation can be stimulated by certain bioactive polypeptides, thus suggesting a novel regulatory role for such compounds in early gonad development.

Original languageEnglish
Pages (from-to)215-221
Number of pages7
JournalDevelopment
Volume122
Issue number1
Publication statusPublished - Jan 1996

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Pituitary Adenylate Cyclase-Activating Polypeptide
Adenylyl Cyclases
Germ Cells
Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
Leukemia Inhibitory Factor
Stem Cell Factor
Secretin
Vasoactive Intestinal Peptide
Gonads
Neuropeptides
Glucagon
Intercellular Signaling Peptides and Proteins
Cell Proliferation
Hormones
Peptides

Keywords

  • Adenylate cyclase
  • Fetal gonad
  • Mouse
  • PACAP
  • Primordial germ cells

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Pituitary adenylate cyclase-activating polypeptide (PACAP) stimulates adenylate cyclase and promotes proliferation of mouse primordial germ cells. / Pesce, Maurizio; Canipari, Rita; Ferri, Gian Luca; Siracusa, Gregorio; De Felici, Massimo.

In: Development, Vol. 122, No. 1, 01.1996, p. 215-221.

Research output: Contribution to journalArticle

Pesce, Maurizio ; Canipari, Rita ; Ferri, Gian Luca ; Siracusa, Gregorio ; De Felici, Massimo. / Pituitary adenylate cyclase-activating polypeptide (PACAP) stimulates adenylate cyclase and promotes proliferation of mouse primordial germ cells. In: Development. 1996 ; Vol. 122, No. 1. pp. 215-221.
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