Pitx2 promotes development of splanchnic mesoderm-derived branchiomeric muscle

Feiyan Dong, Xiaoxia Sun, Wei Liu, Di Ai, Elizabetha Klysik, Mei Fang Lu, Julia Hadley, Laurent Antoni, Li Chen, Antonio Baldini, Pip Francis-West, James F. Martin

Research output: Contribution to journalArticlepeer-review


Recent experiments, showing that both cranial paraxial and splanchnic mesoderm contribute to branchiomeric muscle and cardiac outflow tract (OFT) myocardium, revealed unexpected complexity in development of these muscle groups. The Pitx2 homeobox gene functions in both cranial paraxial mesoderm, to regulate eye muscle, and in splanchnic mesoderm to regulate OFT development. Here, we investigated Pitx2 in branchiomeric muscle. Pitx2 was expressed in branchial arch core mesoderm and both Pitx2 null and Pitx2 hypomorphic embryos had defective branchiomeric muscle. Lineage tracing with a Pitx2cre allele indicated that Pitx2 mutant descendents moved into the first branchial arch. However, markers of both undifferentiated core mesoderm and specified branchiomeric Muscle were absent. Moreover, lineage tracing with a Myf5cre allele indicated that branchiomeric muscle specification and differentiation were defective in Pitx2 mutants. Conditional inactivation in mice and manipulation of Pitx2 expression in chick mandible cultures revealed an autonomous function in expansion and survival of branchial arch mesoderm.

Original languageEnglish
Pages (from-to)4891-4899
Number of pages9
Issue number24
Publication statusPublished - Dec 2006


  • Branchiomeric muscle
  • Chick
  • Homeobox
  • Mouse

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology


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