Pivotal role of the RB family proteins in in vitro thyroid cell transformation

Rodolfo Iuliano, Francesco Trapasso, Antonella Stella, Ilaria Le Pera, Rosa Marina Melillo, Paola Bruni, Gustavo Baldassarre, Lorenzo Chiariotti, Massimo Santoro, Giuseppe Viglietto, Alfredo Fusco

Research output: Contribution to journalArticle

Abstract

Rat thyroid differentiated cells (PC Cl 3) are an excellent model system with which to study the interaction between differentiation and cell transformation. We previously demonstrated that PC Cl 3 cells expressing the adenovirus E1A gene no longer depend on thyrotropin for growth and do not express thyroid differentiation markers. Here we show that an E1A mutant unable to bind the RB protein failed to transform the PC Cl 3 cells. Conversely, mutations in the E1A p300 interacting region did not affect its transforming ability. The pivotal role of RB family proteins in the thyroid cell transformation is supported by the thyrotropin independence induced by the E7 gene of human papilloma virus type 16, but not by a mutated form in the RB-binding region. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)257-267
Number of pages11
JournalExperimental Cell Research
Volume260
Issue number2
DOIs
Publication statusPublished - Nov 1 2000

Keywords

  • E1A
  • E2F
  • E7
  • RB
  • Thyroid
  • Transformation

ASJC Scopus subject areas

  • Cell Biology

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