pKBuS13, a KPC-2-encoding plasmid from Klebsiella pneumoniae sequence type 833, carrying Tn4401b inserted into an Xer site-specific recombination locus

Luigi Garbari, Marina Busetti, Lucilla Dolzani, Vincenzo Petix, Anna Knezevich, Raffaela Bressan, Fabrizia Gionechetti, Enrico A. Tonin, Cristina Lagatolla

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Here, we report the first detection of a Klebsiella pneumoniae carbapenemase 2 (KPC-2)-producing Klebsiella pneumoniae strain belonging to sequence type 833 (ST833), collected in an Italian hospital from a patient coming from South America. Its blaKPC determinant was carried by a ColE1 plasmid, pKBuS13, that showed the Tn4401b::blaKPC-2 transposon inserted into the regulatory region of an Xer site-specific recombination locus. This interfered with the correct resolution of plasmid multimers into monomers, lowering plasmid stability and leading to overestimation of the number of plasmids harbored by a single host cell. Sequencing of the fragments adjacent to Tn4401b detected a region that did not have significant matches in databases other than the genome of a carbapenem-resistant Escherichia coli strain collected during the same year at a hospital in Boston. This is interesting in an epidemiologic context, as it suggests that despite the absence of tra genes and the instability under nonselective conditions, the circulation of pKBuS13 or of analogous plasmids might be wider than reported.

Original languageEnglish
Pages (from-to)5226-5231
Number of pages6
JournalAntimicrobial Agents and Chemotherapy
Volume59
Issue number9
DOIs
Publication statusPublished - Sep 1 2015

Fingerprint

Klebsiella pneumoniae
Genetic Recombination
Plasmids
Carbapenems
South America
Nucleic Acid Regulatory Sequences
Klebsiella pneumoniae carbapenemase-2
Genome
Databases
Escherichia coli
Genes

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology
  • Infectious Diseases

Cite this

pKBuS13, a KPC-2-encoding plasmid from Klebsiella pneumoniae sequence type 833, carrying Tn4401b inserted into an Xer site-specific recombination locus. / Garbari, Luigi; Busetti, Marina; Dolzani, Lucilla; Petix, Vincenzo; Knezevich, Anna; Bressan, Raffaela; Gionechetti, Fabrizia; Tonin, Enrico A.; Lagatolla, Cristina.

In: Antimicrobial Agents and Chemotherapy, Vol. 59, No. 9, 01.09.2015, p. 5226-5231.

Research output: Contribution to journalArticle

Garbari, Luigi ; Busetti, Marina ; Dolzani, Lucilla ; Petix, Vincenzo ; Knezevich, Anna ; Bressan, Raffaela ; Gionechetti, Fabrizia ; Tonin, Enrico A. ; Lagatolla, Cristina. / pKBuS13, a KPC-2-encoding plasmid from Klebsiella pneumoniae sequence type 833, carrying Tn4401b inserted into an Xer site-specific recombination locus. In: Antimicrobial Agents and Chemotherapy. 2015 ; Vol. 59, No. 9. pp. 5226-5231.
@article{7ab88051d5654e39a7f58d61069500c1,
title = "pKBuS13, a KPC-2-encoding plasmid from Klebsiella pneumoniae sequence type 833, carrying Tn4401b inserted into an Xer site-specific recombination locus",
abstract = "Here, we report the first detection of a Klebsiella pneumoniae carbapenemase 2 (KPC-2)-producing Klebsiella pneumoniae strain belonging to sequence type 833 (ST833), collected in an Italian hospital from a patient coming from South America. Its blaKPC determinant was carried by a ColE1 plasmid, pKBuS13, that showed the Tn4401b::blaKPC-2 transposon inserted into the regulatory region of an Xer site-specific recombination locus. This interfered with the correct resolution of plasmid multimers into monomers, lowering plasmid stability and leading to overestimation of the number of plasmids harbored by a single host cell. Sequencing of the fragments adjacent to Tn4401b detected a region that did not have significant matches in databases other than the genome of a carbapenem-resistant Escherichia coli strain collected during the same year at a hospital in Boston. This is interesting in an epidemiologic context, as it suggests that despite the absence of tra genes and the instability under nonselective conditions, the circulation of pKBuS13 or of analogous plasmids might be wider than reported.",
author = "Luigi Garbari and Marina Busetti and Lucilla Dolzani and Vincenzo Petix and Anna Knezevich and Raffaela Bressan and Fabrizia Gionechetti and Tonin, {Enrico A.} and Cristina Lagatolla",
year = "2015",
month = "9",
day = "1",
doi = "10.1128/AAC.04543-14",
language = "English",
volume = "59",
pages = "5226--5231",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
number = "9",

}

TY - JOUR

T1 - pKBuS13, a KPC-2-encoding plasmid from Klebsiella pneumoniae sequence type 833, carrying Tn4401b inserted into an Xer site-specific recombination locus

AU - Garbari, Luigi

AU - Busetti, Marina

AU - Dolzani, Lucilla

AU - Petix, Vincenzo

AU - Knezevich, Anna

AU - Bressan, Raffaela

AU - Gionechetti, Fabrizia

AU - Tonin, Enrico A.

AU - Lagatolla, Cristina

PY - 2015/9/1

Y1 - 2015/9/1

N2 - Here, we report the first detection of a Klebsiella pneumoniae carbapenemase 2 (KPC-2)-producing Klebsiella pneumoniae strain belonging to sequence type 833 (ST833), collected in an Italian hospital from a patient coming from South America. Its blaKPC determinant was carried by a ColE1 plasmid, pKBuS13, that showed the Tn4401b::blaKPC-2 transposon inserted into the regulatory region of an Xer site-specific recombination locus. This interfered with the correct resolution of plasmid multimers into monomers, lowering plasmid stability and leading to overestimation of the number of plasmids harbored by a single host cell. Sequencing of the fragments adjacent to Tn4401b detected a region that did not have significant matches in databases other than the genome of a carbapenem-resistant Escherichia coli strain collected during the same year at a hospital in Boston. This is interesting in an epidemiologic context, as it suggests that despite the absence of tra genes and the instability under nonselective conditions, the circulation of pKBuS13 or of analogous plasmids might be wider than reported.

AB - Here, we report the first detection of a Klebsiella pneumoniae carbapenemase 2 (KPC-2)-producing Klebsiella pneumoniae strain belonging to sequence type 833 (ST833), collected in an Italian hospital from a patient coming from South America. Its blaKPC determinant was carried by a ColE1 plasmid, pKBuS13, that showed the Tn4401b::blaKPC-2 transposon inserted into the regulatory region of an Xer site-specific recombination locus. This interfered with the correct resolution of plasmid multimers into monomers, lowering plasmid stability and leading to overestimation of the number of plasmids harbored by a single host cell. Sequencing of the fragments adjacent to Tn4401b detected a region that did not have significant matches in databases other than the genome of a carbapenem-resistant Escherichia coli strain collected during the same year at a hospital in Boston. This is interesting in an epidemiologic context, as it suggests that despite the absence of tra genes and the instability under nonselective conditions, the circulation of pKBuS13 or of analogous plasmids might be wider than reported.

UR - http://www.scopus.com/inward/record.url?scp=84940934160&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84940934160&partnerID=8YFLogxK

U2 - 10.1128/AAC.04543-14

DO - 10.1128/AAC.04543-14

M3 - Article

VL - 59

SP - 5226

EP - 5231

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 9

ER -