PKC activity in rat C6-glioma cells

Changes associated with cell cycle and simvastatin treatment

M. R. Soma, R. Baetta, S. Bergamaschi, M. R. De Renzis, C. Davegna, F. Battaini, R. Fumagalli, S. Govoni

Research output: Contribution to journalArticle

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Abstract

The parallel effects of simvastatin on cell cycle and PKC activity in rat C6 glioma cells were investigated. Simvastatin, 2.5 μM, for 24 h resulted in cell growth arrest in early G1 phase of the cell cycle and in a significant increase of total PKC activity (283 ± 42 vs 470 ± 61 pmoles/min/mg protein p = 0.002 for control cells and simvastatin-treated cells, respectively). The effect of simvastatin was fully prevented by mevalonate. A time dependent increase of PKC activity was observed in control exponentially free-growing C6 cells approaching confluency: a highly significant negative correlation (r = -0.91, p <0.0001) between PKC activity and growth rate was calculated. PKC activity was high in cells arrested in G0 by serum starvation (0.4%). Following addition of complete medium (17.5% serum) the PKC activity progressively decreased and reached a minimum when cells traversed the G2/M phase, as determined by DNA analysis distribution. PKC activity dropped 30% in simvastatin-arrested early G1 cells; 44% in hydroxyurea-arrested cells at the G1/S boundary; and 73% in Colcemid mitosis-blocked cells. The results show that C6 glioma cell PKC activity is maximal in a G0 quiescent state and varies at different points of the cell cycle.

Original languageEnglish
Pages (from-to)1143-1149
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume200
Issue number2
DOIs
Publication statusPublished - 1994

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Simvastatin
Glioma
Rats
Cell Cycle
Cells
Demecolcine
Mevalonic Acid
Hydroxyurea
Cell growth
DNA
G2 Phase
G1 Phase
Growth
Starvation
Serum
Mitosis
Cell Division
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

PKC activity in rat C6-glioma cells : Changes associated with cell cycle and simvastatin treatment. / Soma, M. R.; Baetta, R.; Bergamaschi, S.; De Renzis, M. R.; Davegna, C.; Battaini, F.; Fumagalli, R.; Govoni, S.

In: Biochemical and Biophysical Research Communications, Vol. 200, No. 2, 1994, p. 1143-1149.

Research output: Contribution to journalArticle

Soma, MR, Baetta, R, Bergamaschi, S, De Renzis, MR, Davegna, C, Battaini, F, Fumagalli, R & Govoni, S 1994, 'PKC activity in rat C6-glioma cells: Changes associated with cell cycle and simvastatin treatment', Biochemical and Biophysical Research Communications, vol. 200, no. 2, pp. 1143-1149. https://doi.org/10.1006/bbrc.1994.1570
Soma, M. R. ; Baetta, R. ; Bergamaschi, S. ; De Renzis, M. R. ; Davegna, C. ; Battaini, F. ; Fumagalli, R. ; Govoni, S. / PKC activity in rat C6-glioma cells : Changes associated with cell cycle and simvastatin treatment. In: Biochemical and Biophysical Research Communications. 1994 ; Vol. 200, No. 2. pp. 1143-1149.
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