PKC activity in rat C6-glioma cells: Changes associated with cell cycle and simvastatin treatment

M. R. Soma, R. Baetta, S. Bergamaschi, M. R. De Renzis, C. Davegna, F. Battaini, R. Fumagalli, S. Govoni

Research output: Contribution to journalArticle

Abstract

The parallel effects of simvastatin on cell cycle and PKC activity in rat C6 glioma cells were investigated. Simvastatin, 2.5 μM, for 24 h resulted in cell growth arrest in early G1 phase of the cell cycle and in a significant increase of total PKC activity (283 ± 42 vs 470 ± 61 pmoles/min/mg protein p = 0.002 for control cells and simvastatin-treated cells, respectively). The effect of simvastatin was fully prevented by mevalonate. A time dependent increase of PKC activity was observed in control exponentially free-growing C6 cells approaching confluency: a highly significant negative correlation (r = -0.91, p <0.0001) between PKC activity and growth rate was calculated. PKC activity was high in cells arrested in G0 by serum starvation (0.4%). Following addition of complete medium (17.5% serum) the PKC activity progressively decreased and reached a minimum when cells traversed the G2/M phase, as determined by DNA analysis distribution. PKC activity dropped 30% in simvastatin-arrested early G1 cells; 44% in hydroxyurea-arrested cells at the G1/S boundary; and 73% in Colcemid mitosis-blocked cells. The results show that C6 glioma cell PKC activity is maximal in a G0 quiescent state and varies at different points of the cell cycle.

Original languageEnglish
Pages (from-to)1143-1149
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume200
Issue number2
DOIs
Publication statusPublished - 1994

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ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Soma, M. R., Baetta, R., Bergamaschi, S., De Renzis, M. R., Davegna, C., Battaini, F., Fumagalli, R., & Govoni, S. (1994). PKC activity in rat C6-glioma cells: Changes associated with cell cycle and simvastatin treatment. Biochemical and Biophysical Research Communications, 200(2), 1143-1149. https://doi.org/10.1006/bbrc.1994.1570