Plant tdp1 (Tyrosyl-dna phosphodiesterase 1): A phylogenetic perspective and gene expression data mining

Giacomo Mutti, Alessandro Raveane, Andrea Pagano, Francesco Bertolini, Ornella Semino, Alma Balestrazzi, Anca Macovei

Research output: Contribution to journalArticlepeer-review

Abstract

The TDP1 (tyrosyl-DNA phosphodiesterase 1) enzyme removes the non-specific covalent intermediates between topoisomerase I and DNA, thus playing a crucial role in preventing DNA damage. While mammals possess only one TDP1 gene, in plants two genes (TDP1α and TDP1β) are present constituting a small gene subfamily. These display a different domain structure and appear to perform non-overlapping functions in the maintenance of genome integrity. Namely, the HIRAN domain identified in TDP1β is involved in the interaction with DNA during the recognition of stalled replication forks. The availability of transcriptomic databases in a growing variety of experimental systems provides new opportunities to fill the current gaps of knowledge concerning the evolutionary origin and the specialized roles of TDP1 genes in plants. Whereas a phylogenetic approach has been used to track the evolution of plant TDP1 protein, transcriptomic data from a selection of representative lycophyte, eudicots, and monocots have been implemented to explore the transcriptomic dynamics in different tissues and a variety of biotic and abiotic stress conditions. While the phylogenetic analysis indicates that TDP1α is of non-plant origin and TDP1β is plant-specific originating in ancient vascular plants, the gene expression data mining comparative analysis pinpoints for tissue-and stress-specific responses.

Original languageEnglish
Article number1465
Pages (from-to)1-22
Number of pages22
JournalGenes
Volume11
Issue number12
DOIs
Publication statusPublished - Dec 2020

Keywords

  • DNA damage
  • Expression profiling
  • Phylogenetic analysis
  • Sequence organization
  • Tyrosyl-DNA phosphodiesterase

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Fingerprint

Dive into the research topics of 'Plant tdp1 (Tyrosyl-dna phosphodiesterase 1): A phylogenetic perspective and gene expression data mining'. Together they form a unique fingerprint.

Cite this