Plaque assay with protein A-coated erythrocytes for the evaluation of human immunoglobulin-secreting cells induced by pokeweed mitogen

A. Lanzavecchia, G. Sacchi, R. Maccario, A. Vitiello, L. Nespoli, A. G. Ugazio

Research output: Contribution to journalArticlepeer-review

Abstract

Cells secreting IgM, IgG and IgA were evaluated in cultures of human peripheral blood lymphocytes stimulated in vitro with pokeweed mitogen by a hemolytic plaque assay using protein A-coated erythrocytes in the presence of class-specific antisera. Kinetic study revealed that immunoglobulin-secreting cells appeared after 3 days of culture and peaked between days 5 and 7. IgM-secreting cells predominated throughout the culture period. This plaque assay is a useful and sensitive in vitro test for the evaluation of polyclonal B-cell activation in humans and may provide a good approach to the study of disturbances in the synthesis of the various classes of immunoglobulins.

Original languageEnglish
Pages (from-to)297-302
Number of pages6
JournalScandinavian Journal of Immunology
Volume10
Issue number4
DOIs
Publication statusPublished - 1979

ASJC Scopus subject areas

  • Immunology

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