Plasma autoantibodies against heat shock protein 70, enolase 1 and ribonuclease/angiogenin inhibitor 1 as potential biomarkers for cholangiocarcinoma

Rucksak Rucksaken, Chawalit Pairojkul, Porntip Pinlaor, Narong Khuntikeo, Sittiruk Roytrakul, Carlo Selmi, Somchai Pinlaor

Research output: Contribution to journalArticle

Abstract

The diagnosis of cholangiocarcinoma (CCA) is often challenging, leading to poor prognosis. CCA arises via chronic inflammation which may be associated with autoantibodies production. This study aims to identify IgG antibodies directed at self-proteins and tumor-associated antigens. Proteins derived from immortalized cholangiocyte cell line (MMNK1) and CCA cell lines (M055, M214 and M139) were separated using 2-dimensional electrophoresis and incubated with pooled plasma of patients with CCA and non-neoplastic controls by immunoblotting. Twenty five immunoreactive spots against all cell lines-derived proteins were observed on stained gels and studied by LC-MS/MS. Among these, heat shock protein 70 (HSP70), enolase 1 (ENO1) and ribonuclease/angiogenin inhibitor 1 (RNH1) obtained the highest matching scores and were thus selected for further validation. Western blot revealed immunoreactivity against HSP70 and RNH1 in the majority of CCA cases and weakly in healthy individuals. Further, ELISA showed that plasma HSP70 autoantibody level in CCA was significantly capable to discriminate CCA from healthy individuals with an area under the receiver operating characteristic curve of 0.9158 (cut-off 0.2630, 93.55% sensitivity and 73.91% specificity). Plasma levels of IgG autoantibodies against HSP70 were correlated with progression from healthy individuals to cholangitis to CCA (r = 0.679, P

Original languageEnglish
Article numbere103259
JournalPLoS One
Volume9
Issue number7
DOIs
Publication statusPublished - Jul 24 2014

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phosphopyruvate hydratase
autoantibodies
HSP70 Heat-Shock Proteins
Cholangiocarcinoma
ribonucleases
Phosphopyruvate Hydratase
Biomarkers
Autoantibodies
biomarkers
Plasmas
Cells
cell lines
Immunoglobulin G
Proteins
proteins
antibody formation
immunoblotting
Electrophoresis
Cell Line
prognosis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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Plasma autoantibodies against heat shock protein 70, enolase 1 and ribonuclease/angiogenin inhibitor 1 as potential biomarkers for cholangiocarcinoma. / Rucksaken, Rucksak; Pairojkul, Chawalit; Pinlaor, Porntip; Khuntikeo, Narong; Roytrakul, Sittiruk; Selmi, Carlo; Pinlaor, Somchai.

In: PLoS One, Vol. 9, No. 7, e103259, 24.07.2014.

Research output: Contribution to journalArticle

Rucksaken, Rucksak ; Pairojkul, Chawalit ; Pinlaor, Porntip ; Khuntikeo, Narong ; Roytrakul, Sittiruk ; Selmi, Carlo ; Pinlaor, Somchai. / Plasma autoantibodies against heat shock protein 70, enolase 1 and ribonuclease/angiogenin inhibitor 1 as potential biomarkers for cholangiocarcinoma. In: PLoS One. 2014 ; Vol. 9, No. 7.
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