Plasma Cell-Free DNA in Paediatric Lymphomas

Lara Mussolin, Roberta Burnelli, Marta Pillon, Elisa Carraro, Piero Farruggia, Alessandra Todesco, Maurizio Mascarin, Angelo Rosolen

Research output: Contribution to journalArticle

Abstract

Background: Extracellular circulating DNA (cfDNA) can be found in small amounts in plasm of healthy individuals. Increased levels of cfDNA have been reported in patients with cancer of breast, cervix, colon, liver and it was shown that cfDNA can originate from both tumour an non-tumour cells. Objectives: Levels of cfDNA of a large series of children with lymphoma were evaluated and analyzed in relation with clinical characteristics. Methods: plasma cfDNA levels obtained at diagnosis in 201 paediatric lymphoma patients [43 Hodgkin lymphomas (HL), 45 anaplastic large cell lymphomas (ALCL), 88 Burkitt lymphomas (BL), 17 lymphoblastic (LBL), 8 diffuse large B cell lymphoma (DLBCL)] and 15 healthy individuals were determined using a quantitative PCR assay for POLR2 gene and, in addition, for NPM-ALK fusion gene in ALCL patients. Wilcoxon rank sum test was used to compare plasma levels among dif-ferent patient subgroups and controls and to analyze relationship between levels of cfDNA and clinical characteristics. Results: Levels of cfDNA in lymphoma patients were significantly higher compared with controls (p

Original languageEnglish
Pages (from-to)323-329
Number of pages7
JournalJournal of Cancer
Volume4
Issue number4
DOIs
Publication statusPublished - 2013

Keywords

  • CfDNA
  • Childhood
  • Hodgkin
  • Lymphoma
  • Prognosis

ASJC Scopus subject areas

  • Oncology

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    Mussolin, L., Burnelli, R., Pillon, M., Carraro, E., Farruggia, P., Todesco, A., Mascarin, M., & Rosolen, A. (2013). Plasma Cell-Free DNA in Paediatric Lymphomas. Journal of Cancer, 4(4), 323-329. https://doi.org/10.7150/jca.6226