Plasma circulating miR-23~27~24 clusters correlate with the immunometabolic derangement and predict C-peptide loss in children with type 1 diabetes

S. Garavelli, S. Bruzzaniti, E. Tagliabue, D. Di Silvestre, F. Prattichizzo, E. Mozzillo, V. Fattorusso, L. La Sala, A. Ceriello, A.A. Puca, P. Mauri, R. Strollo, M. Marigliano, C. Maffeis, A. Petrelli, E. Bosi, A. Franzese, M. Galgani, G. Matarese, P. de Candia

Research output: Contribution to journalArticlepeer-review

Abstract

Aims/hypothesis: We aimed to analyse the association between plasma circulating microRNAs (miRNAs) and the immunometabolic profile in children with type 1 diabetes and to identify a composite signature of miRNAs/immunometabolic factors able to predict type 1 diabetes progression. Methods: Plasma samples were obtained from children at diagnosis of type 1 diabetes (n = 88) and at 12 (n = 32) and 24 (n = 30) months after disease onset and from healthy control children with similar sex and age distribution (n = 47). We quantified 60 robustly expressed plasma circulating miRNAs by quantitative RT-PCR and nine plasma immunometabolic factors with a recognised role at the interface of metabolic and immune alterations in type 1 diabetes. Based on fasting C-peptide loss over time, children with type 1 diabetes were stratified into the following groups: those who had lost >90% of C-peptide compared with diagnosis level; those who had lost
Original languageEnglish
Pages (from-to)2699-2712
Number of pages14
JournalDiabetologia
Volume63
Issue number12
DOIs
Publication statusPublished - 2020

Keywords

  • Biomarkers
  • Cardiovascular risk
  • Diabetic complications
  • Immunometabolism
  • Insulin secretion
  • microRNA
  • Osteoprotegerin
  • biological marker
  • C peptide
  • circulating microRNA
  • cytokine receptor
  • hormone receptor
  • insulin
  • leptin
  • lymphocyte antigen
  • microRNA 23
  • microRNA 23a 3p
  • microRNA 23b 3p
  • microRNA 24
  • microRNA 24 3p
  • microRNA 27
  • microRNA 27a 3p
  • microRNA 27b 3p
  • monocyte chemotactic protein 1
  • myeloperoxidase
  • osteoprotegerin
  • resistin
  • soluble cd40 ligand
  • soluble intercellular adhesion molecule 1
  • soluble leptin receptor
  • soluble tumor necrosis factor receptor
  • unclassified drug
  • age distribution
  • Article
  • artificial neural network
  • child
  • clinical evaluation
  • cohort analysis
  • comparative study
  • controlled study
  • correlation coefficient
  • disease association
  • disease duration
  • disease exacerbation
  • fasting
  • female
  • gene expression level
  • gene expression regulation
  • human
  • immune dysregulation
  • immunomodulation
  • immunopathology
  • independent variable
  • inflammation
  • insulin dependent diabetes mellitus
  • insulin release
  • insulin treatment
  • major clinical study
  • male
  • metabolic disorder
  • pediatric patient
  • plasma
  • predictive value
  • priority journal
  • prognosis
  • real time polymerase chain reaction
  • retrospective study
  • school child
  • sex ratio
  • signal transduction
  • upregulation
  • validation study

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