Abstract
Objectives: Oxidative stress caused by acute hyperhomocysteinemia impairs endothelial function in human arteries. We sought to identify markers of endothelial dysfunction during methionine-induced hyperhomocysteinemia. Design and methods: 35 subjects underwent flow-mediated dilation (FMD) of the brachial artery by high-resolution ultrasonography and fasting blood samples before and 3 h postmethionine load (PML). Clinical, conventional biochemical, and redox status (plasma total and reduced homocysteine, glutathione, cysteine, cysteinylglycine, ascorbic acid, α-tocopherol, free malondialdehyde, blood glutathione) data were sequentially entered into an univariate and multivariate stepwise linear regression analysis to evaluate their relation with the dependent variable FMD. Results: Median [interquartile range] FMD decreased from 4.1% [2.8-6.3] to 3.2% [0.7-4.3] PML (P = 0.02). At the multivariate analysis PML total cysteine (β = - 0.008, P = 0.002) and glutathione (β = 0.21, P = 0.005) were the only independent variables associated with FMD after methionine, adjusted for baseline FMD. Conclusions: Elevated plasma total cysteine and decreased plasma total glutathione levels were associated with abnormal FMD PML. Cysteine and glutathione are stronger markers of endothelial dysfunction than clinical and all other biochemical variables explored.
| Original language | English |
|---|---|
| Pages (from-to) | 188-193 |
| Number of pages | 6 |
| Journal | Clinical Biochemistry |
| Volume | 40 |
| Issue number | 3-4 |
| DOIs | |
| Publication status | Published - Feb 2007 |
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Keywords
- Antioxidants
- Endothelial function
- Flow-mediated vasodilation
- Homocysteine
ASJC Scopus subject areas
- Clinical Biochemistry
Cite this
Plasma cysteine and glutathione are independent markers of postmethionine load endothelial dysfunction. / Parodi, Oberdan; De Chiara, Benedetta; Baldassarre, Damiano; Parolini, Marina; Caruso, Raffaele; Pustina, Linda; Parodi, Guido; Campolo, Jonica; Sedda, Valentina; Baudo, Francesco; Sirtori, Cesare.
In: Clinical Biochemistry, Vol. 40, No. 3-4, 02.2007, p. 188-193.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Plasma cysteine and glutathione are independent markers of postmethionine load endothelial dysfunction
AU - Parodi, Oberdan
AU - De Chiara, Benedetta
AU - Baldassarre, Damiano
AU - Parolini, Marina
AU - Caruso, Raffaele
AU - Pustina, Linda
AU - Parodi, Guido
AU - Campolo, Jonica
AU - Sedda, Valentina
AU - Baudo, Francesco
AU - Sirtori, Cesare
PY - 2007/2
Y1 - 2007/2
N2 - Objectives: Oxidative stress caused by acute hyperhomocysteinemia impairs endothelial function in human arteries. We sought to identify markers of endothelial dysfunction during methionine-induced hyperhomocysteinemia. Design and methods: 35 subjects underwent flow-mediated dilation (FMD) of the brachial artery by high-resolution ultrasonography and fasting blood samples before and 3 h postmethionine load (PML). Clinical, conventional biochemical, and redox status (plasma total and reduced homocysteine, glutathione, cysteine, cysteinylglycine, ascorbic acid, α-tocopherol, free malondialdehyde, blood glutathione) data were sequentially entered into an univariate and multivariate stepwise linear regression analysis to evaluate their relation with the dependent variable FMD. Results: Median [interquartile range] FMD decreased from 4.1% [2.8-6.3] to 3.2% [0.7-4.3] PML (P = 0.02). At the multivariate analysis PML total cysteine (β = - 0.008, P = 0.002) and glutathione (β = 0.21, P = 0.005) were the only independent variables associated with FMD after methionine, adjusted for baseline FMD. Conclusions: Elevated plasma total cysteine and decreased plasma total glutathione levels were associated with abnormal FMD PML. Cysteine and glutathione are stronger markers of endothelial dysfunction than clinical and all other biochemical variables explored.
AB - Objectives: Oxidative stress caused by acute hyperhomocysteinemia impairs endothelial function in human arteries. We sought to identify markers of endothelial dysfunction during methionine-induced hyperhomocysteinemia. Design and methods: 35 subjects underwent flow-mediated dilation (FMD) of the brachial artery by high-resolution ultrasonography and fasting blood samples before and 3 h postmethionine load (PML). Clinical, conventional biochemical, and redox status (plasma total and reduced homocysteine, glutathione, cysteine, cysteinylglycine, ascorbic acid, α-tocopherol, free malondialdehyde, blood glutathione) data were sequentially entered into an univariate and multivariate stepwise linear regression analysis to evaluate their relation with the dependent variable FMD. Results: Median [interquartile range] FMD decreased from 4.1% [2.8-6.3] to 3.2% [0.7-4.3] PML (P = 0.02). At the multivariate analysis PML total cysteine (β = - 0.008, P = 0.002) and glutathione (β = 0.21, P = 0.005) were the only independent variables associated with FMD after methionine, adjusted for baseline FMD. Conclusions: Elevated plasma total cysteine and decreased plasma total glutathione levels were associated with abnormal FMD PML. Cysteine and glutathione are stronger markers of endothelial dysfunction than clinical and all other biochemical variables explored.
KW - Antioxidants
KW - Endothelial function
KW - Flow-mediated vasodilation
KW - Homocysteine
UR - http://www.scopus.com/inward/record.url?scp=33846258176&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33846258176&partnerID=8YFLogxK
U2 - 10.1016/j.clinbiochem.2006.08.017
DO - 10.1016/j.clinbiochem.2006.08.017
M3 - Article
VL - 40
SP - 188
EP - 193
JO - Clinical Biochemistry
JF - Clinical Biochemistry
SN - 0009-9120
IS - 3-4
ER -