Plasma cysteine and glutathione are independent markers of postmethionine load endothelial dysfunction

Oberdan Parodi, Benedetta De Chiara, Damiano Baldassarre, Marina Parolini, Raffaele Caruso, Linda Pustina, Guido Parodi, Jonica Campolo, Valentina Sedda, Francesco Baudo, Cesare Sirtori

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives: Oxidative stress caused by acute hyperhomocysteinemia impairs endothelial function in human arteries. We sought to identify markers of endothelial dysfunction during methionine-induced hyperhomocysteinemia. Design and methods: 35 subjects underwent flow-mediated dilation (FMD) of the brachial artery by high-resolution ultrasonography and fasting blood samples before and 3 h postmethionine load (PML). Clinical, conventional biochemical, and redox status (plasma total and reduced homocysteine, glutathione, cysteine, cysteinylglycine, ascorbic acid, α-tocopherol, free malondialdehyde, blood glutathione) data were sequentially entered into an univariate and multivariate stepwise linear regression analysis to evaluate their relation with the dependent variable FMD. Results: Median [interquartile range] FMD decreased from 4.1% [2.8-6.3] to 3.2% [0.7-4.3] PML (P = 0.02). At the multivariate analysis PML total cysteine (β = - 0.008, P = 0.002) and glutathione (β = 0.21, P = 0.005) were the only independent variables associated with FMD after methionine, adjusted for baseline FMD. Conclusions: Elevated plasma total cysteine and decreased plasma total glutathione levels were associated with abnormal FMD PML. Cysteine and glutathione are stronger markers of endothelial dysfunction than clinical and all other biochemical variables explored.

Original languageEnglish
Pages (from-to)188-193
Number of pages6
JournalClinical Biochemistry
Volume40
Issue number3-4
DOIs
Publication statusPublished - Feb 2007

Keywords

  • Antioxidants
  • Endothelial function
  • Flow-mediated vasodilation
  • Homocysteine

ASJC Scopus subject areas

  • Clinical Biochemistry

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