Plasma levels of interleukin-6 and interleukin-10 in preterm neonates evaluated for sepsis

Costantino Romagnoli, Simonetta Frezza, Antonella Cingolani, Andrea De Luca, Maria Puopolo, Maria Pia De Carolis, Giovanni Vento, Andrea Antinori, Giuseppe Tortorolo

Research output: Contribution to journalArticle

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Abstract

In a prospective study, plasma interleukin-6 (IL-6) and interleukin-10 (IL-10) levels were measured by enzyme-linked immunosorbent assay in 45 premature neonates (25-34 weeks gestational age) with signs and symptoms of suspected sepsis at 0, 12 and 24 h; C-reactive protein (CRP) was measured at 0-24 h after enrolment. Six subjects were excluded due to insufficient blood sampling. The remaining 39 neonates were assigned to one of three groups: 25 newborns with sepsis (blood culture positive), seven with pneumonia (positive results on broncho-alveolar lavage fluid culture and characteristic chest radiography) and seven with necrotising enterocolitis (NEC) (characteristic intestinal and radiological signs according to the criteria of Bell et al.). A group of 20 healthy preterm neonates represented control subjects. On admission, higher levels of IL-6, IL-10 and CRP were observed in neonates with sepsis: IL-6 (median 1500 pg/ml, range 487-10000 pg/ml), IL-10 (median 113 pg/ml, range 70-196 pg/ml), CRP (median 22 mg/l, range 4-80 mg/1); pneumonia: IL-6 (median 1500 pg/ml, range 747-8000 pg/ml, IL-10 (median 84 pg/ml, range 76-92 pg/ml), CRP (median 10 mg/1, range 8-33 mg/1) and NEC: IL-6 (median 6650 pg/ml, range 1595-7950 pg/ml), IL-10 (median 80 pg/ml, range 61-147 pg/ml), CRP (median 3 mg/1, range 2.8-8 mg/l) as compared to controls (IL-6 median 208 pg/ml, range 198-349 pg/ml; IL-10 median 36 pg/ml, range 19-50 pg/ml; CRP median <2 mg/1) (P <0.05). In neonates with sepsis, IL-6 levels were significantly correlated with IL-10 levels (r = 0.65; P = 0.04) at the time of the second sample. The highest IL-6 levels were observed at onset, while IL-10 was predominant 12 h later. On admission, IL-10 and CRP levels were significantly higher in non-survivors (IL-10 median 507 pg/ml, range 422-753 pg/ml; CRP median 123 mg/1, range 20-219 mg/l) than in survivors (IL-10 median 76 pg/ml, range 61-143 pg/ml; CRP median 8 mg/1 range 3-46 mg/1), while IL-10 levels were significantly higher (P <0.05) also 12 h after admission (non-survivors: IL-10 median 600 pg/ml, range 538-800 pg/ml; survivors: IL-10 median 74 pg/ml, range 53-161 pg/ml). IL-6 and IL-10 levels were significantly correlated with CRP levels on admission (r = 0.45; P = 0.05). Conclusion: Preterm neonates with sepsis, pneumonia or necrotising enterocolitis showed increased interleukin-6, interleukin-10 and C-reactive protein levels. High interleukin-10 concentration was associated with mortality and could be an early indicator of prognosis.

Original languageEnglish
Pages (from-to)345-350
Number of pages6
JournalEuropean Journal of Pediatrics
Volume160
Issue number6
Publication statusPublished - 2001

Fingerprint

Interleukin-10
Interleukin-6
Sepsis
Newborn Infant
C-Reactive Protein
Necrotizing Enterocolitis
Pneumonia
Survivors
Bronchoalveolar Lavage Fluid
Radiography
Gestational Age
Signs and Symptoms

Keywords

  • Interleukin-10
  • Iterleukin-6
  • Necrotising enterocolitis
  • Preterm neonates
  • Sepsis

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Romagnoli, C., Frezza, S., Cingolani, A., De Luca, A., Puopolo, M., De Carolis, M. P., ... Tortorolo, G. (2001). Plasma levels of interleukin-6 and interleukin-10 in preterm neonates evaluated for sepsis. European Journal of Pediatrics, 160(6), 345-350.

Plasma levels of interleukin-6 and interleukin-10 in preterm neonates evaluated for sepsis. / Romagnoli, Costantino; Frezza, Simonetta; Cingolani, Antonella; De Luca, Andrea; Puopolo, Maria; De Carolis, Maria Pia; Vento, Giovanni; Antinori, Andrea; Tortorolo, Giuseppe.

In: European Journal of Pediatrics, Vol. 160, No. 6, 2001, p. 345-350.

Research output: Contribution to journalArticle

Romagnoli, C, Frezza, S, Cingolani, A, De Luca, A, Puopolo, M, De Carolis, MP, Vento, G, Antinori, A & Tortorolo, G 2001, 'Plasma levels of interleukin-6 and interleukin-10 in preterm neonates evaluated for sepsis', European Journal of Pediatrics, vol. 160, no. 6, pp. 345-350.
Romagnoli C, Frezza S, Cingolani A, De Luca A, Puopolo M, De Carolis MP et al. Plasma levels of interleukin-6 and interleukin-10 in preterm neonates evaluated for sepsis. European Journal of Pediatrics. 2001;160(6):345-350.
Romagnoli, Costantino ; Frezza, Simonetta ; Cingolani, Antonella ; De Luca, Andrea ; Puopolo, Maria ; De Carolis, Maria Pia ; Vento, Giovanni ; Antinori, Andrea ; Tortorolo, Giuseppe. / Plasma levels of interleukin-6 and interleukin-10 in preterm neonates evaluated for sepsis. In: European Journal of Pediatrics. 2001 ; Vol. 160, No. 6. pp. 345-350.
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TY - JOUR

T1 - Plasma levels of interleukin-6 and interleukin-10 in preterm neonates evaluated for sepsis

AU - Romagnoli, Costantino

AU - Frezza, Simonetta

AU - Cingolani, Antonella

AU - De Luca, Andrea

AU - Puopolo, Maria

AU - De Carolis, Maria Pia

AU - Vento, Giovanni

AU - Antinori, Andrea

AU - Tortorolo, Giuseppe

PY - 2001

Y1 - 2001

N2 - In a prospective study, plasma interleukin-6 (IL-6) and interleukin-10 (IL-10) levels were measured by enzyme-linked immunosorbent assay in 45 premature neonates (25-34 weeks gestational age) with signs and symptoms of suspected sepsis at 0, 12 and 24 h; C-reactive protein (CRP) was measured at 0-24 h after enrolment. Six subjects were excluded due to insufficient blood sampling. The remaining 39 neonates were assigned to one of three groups: 25 newborns with sepsis (blood culture positive), seven with pneumonia (positive results on broncho-alveolar lavage fluid culture and characteristic chest radiography) and seven with necrotising enterocolitis (NEC) (characteristic intestinal and radiological signs according to the criteria of Bell et al.). A group of 20 healthy preterm neonates represented control subjects. On admission, higher levels of IL-6, IL-10 and CRP were observed in neonates with sepsis: IL-6 (median 1500 pg/ml, range 487-10000 pg/ml), IL-10 (median 113 pg/ml, range 70-196 pg/ml), CRP (median 22 mg/l, range 4-80 mg/1); pneumonia: IL-6 (median 1500 pg/ml, range 747-8000 pg/ml, IL-10 (median 84 pg/ml, range 76-92 pg/ml), CRP (median 10 mg/1, range 8-33 mg/1) and NEC: IL-6 (median 6650 pg/ml, range 1595-7950 pg/ml), IL-10 (median 80 pg/ml, range 61-147 pg/ml), CRP (median 3 mg/1, range 2.8-8 mg/l) as compared to controls (IL-6 median 208 pg/ml, range 198-349 pg/ml; IL-10 median 36 pg/ml, range 19-50 pg/ml; CRP median <2 mg/1) (P <0.05). In neonates with sepsis, IL-6 levels were significantly correlated with IL-10 levels (r = 0.65; P = 0.04) at the time of the second sample. The highest IL-6 levels were observed at onset, while IL-10 was predominant 12 h later. On admission, IL-10 and CRP levels were significantly higher in non-survivors (IL-10 median 507 pg/ml, range 422-753 pg/ml; CRP median 123 mg/1, range 20-219 mg/l) than in survivors (IL-10 median 76 pg/ml, range 61-143 pg/ml; CRP median 8 mg/1 range 3-46 mg/1), while IL-10 levels were significantly higher (P <0.05) also 12 h after admission (non-survivors: IL-10 median 600 pg/ml, range 538-800 pg/ml; survivors: IL-10 median 74 pg/ml, range 53-161 pg/ml). IL-6 and IL-10 levels were significantly correlated with CRP levels on admission (r = 0.45; P = 0.05). Conclusion: Preterm neonates with sepsis, pneumonia or necrotising enterocolitis showed increased interleukin-6, interleukin-10 and C-reactive protein levels. High interleukin-10 concentration was associated with mortality and could be an early indicator of prognosis.

AB - In a prospective study, plasma interleukin-6 (IL-6) and interleukin-10 (IL-10) levels were measured by enzyme-linked immunosorbent assay in 45 premature neonates (25-34 weeks gestational age) with signs and symptoms of suspected sepsis at 0, 12 and 24 h; C-reactive protein (CRP) was measured at 0-24 h after enrolment. Six subjects were excluded due to insufficient blood sampling. The remaining 39 neonates were assigned to one of three groups: 25 newborns with sepsis (blood culture positive), seven with pneumonia (positive results on broncho-alveolar lavage fluid culture and characteristic chest radiography) and seven with necrotising enterocolitis (NEC) (characteristic intestinal and radiological signs according to the criteria of Bell et al.). A group of 20 healthy preterm neonates represented control subjects. On admission, higher levels of IL-6, IL-10 and CRP were observed in neonates with sepsis: IL-6 (median 1500 pg/ml, range 487-10000 pg/ml), IL-10 (median 113 pg/ml, range 70-196 pg/ml), CRP (median 22 mg/l, range 4-80 mg/1); pneumonia: IL-6 (median 1500 pg/ml, range 747-8000 pg/ml, IL-10 (median 84 pg/ml, range 76-92 pg/ml), CRP (median 10 mg/1, range 8-33 mg/1) and NEC: IL-6 (median 6650 pg/ml, range 1595-7950 pg/ml), IL-10 (median 80 pg/ml, range 61-147 pg/ml), CRP (median 3 mg/1, range 2.8-8 mg/l) as compared to controls (IL-6 median 208 pg/ml, range 198-349 pg/ml; IL-10 median 36 pg/ml, range 19-50 pg/ml; CRP median <2 mg/1) (P <0.05). In neonates with sepsis, IL-6 levels were significantly correlated with IL-10 levels (r = 0.65; P = 0.04) at the time of the second sample. The highest IL-6 levels were observed at onset, while IL-10 was predominant 12 h later. On admission, IL-10 and CRP levels were significantly higher in non-survivors (IL-10 median 507 pg/ml, range 422-753 pg/ml; CRP median 123 mg/1, range 20-219 mg/l) than in survivors (IL-10 median 76 pg/ml, range 61-143 pg/ml; CRP median 8 mg/1 range 3-46 mg/1), while IL-10 levels were significantly higher (P <0.05) also 12 h after admission (non-survivors: IL-10 median 600 pg/ml, range 538-800 pg/ml; survivors: IL-10 median 74 pg/ml, range 53-161 pg/ml). IL-6 and IL-10 levels were significantly correlated with CRP levels on admission (r = 0.45; P = 0.05). Conclusion: Preterm neonates with sepsis, pneumonia or necrotising enterocolitis showed increased interleukin-6, interleukin-10 and C-reactive protein levels. High interleukin-10 concentration was associated with mortality and could be an early indicator of prognosis.

KW - Interleukin-10

KW - Iterleukin-6

KW - Necrotising enterocolitis

KW - Preterm neonates

KW - Sepsis

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