Plasma levels of soluble CD30, tumour necrosis factor (TNF)-α and TNF receptors during primary HIV-1 infection: Correlation with HIV-1 RNA and the clinical outcome

G. Paolo Rizzardi, Wilma Barcellini, Giuseppe Tambussi, Flavia Lillo, Mauro Malnati, Luc Perrin, Adriano Lazzarin

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives: The immunological and virological events associated with primary HIV-1 infection have a major impact on the course of HIV-1 disease, and the identification of early predictors during primary HIV infection is critical for the therapeutic strategy. Design and methods: Eighteen consecutive patients with primary HIV infection were followed for a median of 398 days. Clinical status, CD4+ T-cell counts, and plasma samples were obtained weekly from enrolment until week 6, then at weeks 12, 24 and 52, and every 6 months thereafter. Seroconversion was assessed by anti-HIV-1/2 antibodies and Western blot analysis. HIV-1 RNA in plasma was quantified by Amplicor HIV Monitor test. Samples were assayed for immune complex-dissociated p24 antigen, tumour necrosis factor (TNF)-α, soluble TNF receptor (sTNFR)-I, sTNFR-II, sCD30 and sCD8 by enzyme immunoassays. Outcome was defined as entering clinical category B or C according to the Centers for Disease Control and Prevention criteria. As a control group, we included 23 HIV-1-negative healthy blood donors. Results: Plasma levels of sCD30, TNF-α and sTNFR were significantly higher in HIV-1-infected patients than in controls, and were positively correlated with each other and with values of HIV-1 RNA. Patients who developed an outcome (n = 4) had significantly higher levels of sCD30, TNF-α and sTNFR compared with those who did not. Multivariate logistic regression analysis showed that sCD30 and TNF-α were the best predictors of outcome independently of CD4+ T-cell counts. Conclusions: During primary HIV infection, a persistent immune activation may be associated with a poor clinical outcome. The identification of sCD30 and TNF-α levels in plasma as early predictors of outcome in primary HIV infection, may direct the implementation of early therapeutic strategies in patients with elevated risk of disease progression.

Original languageEnglish
JournalAIDS (London, England)
Volume10
Issue number13
Publication statusPublished - 1996

Keywords

  • Acute HIV-1 infection
  • Cellular factors/cytokines
  • Disease progression
  • HIV-1 RNA
  • Soluble CD30
  • Soluble TNF receptors
  • Tumour necrosis factor (TNF)-α

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint

Dive into the research topics of 'Plasma levels of soluble CD30, tumour necrosis factor (TNF)-α and TNF receptors during primary HIV-1 infection: Correlation with HIV-1 RNA and the clinical outcome'. Together they form a unique fingerprint.

Cite this