Plasma levels of vasostatin-1, a chromogranin A fragment, are associated with carotid artery maximum stenosis: A pilot study

Tiziana Bachetti, Alberto Ferrari Bardile, Teresa Lucia Aloi, Barbara Colombo, Emma Assi, Giuseppina Savino, Andrea Vercelli, Roberto Colombo, Angelo Corti

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

BACKGROUND: Chromogranin A (CgA), a circulating protein released by the neuroendocrine system, can regulate vascular physiology and angiogenesis. Full-length CgA (CgA1-439) and its fragment CgA1-76 (called vasostatin-1, VS-1) preserve the physiological integrity of the endothelial barrier function and are antiangiogenic, whereas CgA1-373 is proangiogenic. We investigated whether these polypeptides are altered in patients with various degrees of carotid artery atherosclerosis.

METHODS: We studied 81 patients with carotid artery atherosclerosis, asymptomatic for cerebrovascular diseases. Carotid arteries were examined by Doppler ultrasound and plaque characteristics were recorded. Plasma levels of CgA1-439, VS-1, CgA1-373, and total-CgA (CgA1-439 plus truncated fragments lacking part or the entire C-terminal region) were assessed by specific ELISAs.

RESULTS: Plasma levels of VS-1 and total-CgA correlated with carotid artery maximum stenosis (r=0.349, p=0.001 and r=0.256, p=0.021, respectively). Stepwise multiple regression analysis indicated that VS-1 was a significant predictor of maximum stenosis after adjustment for age, gender, and conventional risk factors for atherosclerosis (regression coefficient=12.42, SE=4.84, p=0.012). In addition, logistic regression analysis indicated that relatively high levels of full-length CgA, but not total-CgA, predict the presence of hypoechoic, lipid-rich plaques (OR=1.47; 95% CI: 1.19-1.81, p=0.0003).

CONCLUSION: VS-1 is independently associated with carotid artery maximum stenosis. Furthermore, full-length CgA is an independent indicator of hypoechoic plaques, likely reflecting initial stages of atherosclerosis. Given the known capability of CgA and VS-1 to regulate vascular function and angiogenesis these polypeptides might play a role in the regulation of atherosclerosis pathophysiology.

Original languageEnglish
Pages (from-to)438-443
Number of pages6
JournalInternational Journal of Cardiology
Volume236
DOIs
Publication statusPublished - Jun 1 2017

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Chromogranin A
Carotid Stenosis
Carotid Arteries
Atherosclerosis
Carotid Artery Diseases
Blood Vessels
Regression Analysis
Cerebrovascular Disorders
Asymptomatic Diseases
Doppler Ultrasonography
Peptides
Neurosecretory Systems
vasostatin I
Pathologic Constriction
Logistic Models
Enzyme-Linked Immunosorbent Assay
Lipids

Keywords

  • Journal Article

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Plasma levels of vasostatin-1, a chromogranin A fragment, are associated with carotid artery maximum stenosis : A pilot study. / Bachetti, Tiziana; Ferrari Bardile, Alberto; Aloi, Teresa Lucia; Colombo, Barbara; Assi, Emma; Savino, Giuseppina; Vercelli, Andrea; Colombo, Roberto; Corti, Angelo.

In: International Journal of Cardiology, Vol. 236, 01.06.2017, p. 438-443.

Research output: Contribution to journalArticle

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title = "Plasma levels of vasostatin-1, a chromogranin A fragment, are associated with carotid artery maximum stenosis: A pilot study",
abstract = "BACKGROUND: Chromogranin A (CgA), a circulating protein released by the neuroendocrine system, can regulate vascular physiology and angiogenesis. Full-length CgA (CgA1-439) and its fragment CgA1-76 (called vasostatin-1, VS-1) preserve the physiological integrity of the endothelial barrier function and are antiangiogenic, whereas CgA1-373 is proangiogenic. We investigated whether these polypeptides are altered in patients with various degrees of carotid artery atherosclerosis.METHODS: We studied 81 patients with carotid artery atherosclerosis, asymptomatic for cerebrovascular diseases. Carotid arteries were examined by Doppler ultrasound and plaque characteristics were recorded. Plasma levels of CgA1-439, VS-1, CgA1-373, and total-CgA (CgA1-439 plus truncated fragments lacking part or the entire C-terminal region) were assessed by specific ELISAs.RESULTS: Plasma levels of VS-1 and total-CgA correlated with carotid artery maximum stenosis (r=0.349, p=0.001 and r=0.256, p=0.021, respectively). Stepwise multiple regression analysis indicated that VS-1 was a significant predictor of maximum stenosis after adjustment for age, gender, and conventional risk factors for atherosclerosis (regression coefficient=12.42, SE=4.84, p=0.012). In addition, logistic regression analysis indicated that relatively high levels of full-length CgA, but not total-CgA, predict the presence of hypoechoic, lipid-rich plaques (OR=1.47; 95{\%} CI: 1.19-1.81, p=0.0003).CONCLUSION: VS-1 is independently associated with carotid artery maximum stenosis. Furthermore, full-length CgA is an independent indicator of hypoechoic plaques, likely reflecting initial stages of atherosclerosis. Given the known capability of CgA and VS-1 to regulate vascular function and angiogenesis these polypeptides might play a role in the regulation of atherosclerosis pathophysiology.",
keywords = "Journal Article",
author = "Tiziana Bachetti and {Ferrari Bardile}, Alberto and Aloi, {Teresa Lucia} and Barbara Colombo and Emma Assi and Giuseppina Savino and Andrea Vercelli and Roberto Colombo and Angelo Corti",
note = "Copyright {\circledC} 2017 Elsevier B.V. All rights reserved.",
year = "2017",
month = "6",
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doi = "10.1016/j.ijcard.2017.02.019",
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TY - JOUR

T1 - Plasma levels of vasostatin-1, a chromogranin A fragment, are associated with carotid artery maximum stenosis

T2 - A pilot study

AU - Bachetti, Tiziana

AU - Ferrari Bardile, Alberto

AU - Aloi, Teresa Lucia

AU - Colombo, Barbara

AU - Assi, Emma

AU - Savino, Giuseppina

AU - Vercelli, Andrea

AU - Colombo, Roberto

AU - Corti, Angelo

N1 - Copyright © 2017 Elsevier B.V. All rights reserved.

PY - 2017/6/1

Y1 - 2017/6/1

N2 - BACKGROUND: Chromogranin A (CgA), a circulating protein released by the neuroendocrine system, can regulate vascular physiology and angiogenesis. Full-length CgA (CgA1-439) and its fragment CgA1-76 (called vasostatin-1, VS-1) preserve the physiological integrity of the endothelial barrier function and are antiangiogenic, whereas CgA1-373 is proangiogenic. We investigated whether these polypeptides are altered in patients with various degrees of carotid artery atherosclerosis.METHODS: We studied 81 patients with carotid artery atherosclerosis, asymptomatic for cerebrovascular diseases. Carotid arteries were examined by Doppler ultrasound and plaque characteristics were recorded. Plasma levels of CgA1-439, VS-1, CgA1-373, and total-CgA (CgA1-439 plus truncated fragments lacking part or the entire C-terminal region) were assessed by specific ELISAs.RESULTS: Plasma levels of VS-1 and total-CgA correlated with carotid artery maximum stenosis (r=0.349, p=0.001 and r=0.256, p=0.021, respectively). Stepwise multiple regression analysis indicated that VS-1 was a significant predictor of maximum stenosis after adjustment for age, gender, and conventional risk factors for atherosclerosis (regression coefficient=12.42, SE=4.84, p=0.012). In addition, logistic regression analysis indicated that relatively high levels of full-length CgA, but not total-CgA, predict the presence of hypoechoic, lipid-rich plaques (OR=1.47; 95% CI: 1.19-1.81, p=0.0003).CONCLUSION: VS-1 is independently associated with carotid artery maximum stenosis. Furthermore, full-length CgA is an independent indicator of hypoechoic plaques, likely reflecting initial stages of atherosclerosis. Given the known capability of CgA and VS-1 to regulate vascular function and angiogenesis these polypeptides might play a role in the regulation of atherosclerosis pathophysiology.

AB - BACKGROUND: Chromogranin A (CgA), a circulating protein released by the neuroendocrine system, can regulate vascular physiology and angiogenesis. Full-length CgA (CgA1-439) and its fragment CgA1-76 (called vasostatin-1, VS-1) preserve the physiological integrity of the endothelial barrier function and are antiangiogenic, whereas CgA1-373 is proangiogenic. We investigated whether these polypeptides are altered in patients with various degrees of carotid artery atherosclerosis.METHODS: We studied 81 patients with carotid artery atherosclerosis, asymptomatic for cerebrovascular diseases. Carotid arteries were examined by Doppler ultrasound and plaque characteristics were recorded. Plasma levels of CgA1-439, VS-1, CgA1-373, and total-CgA (CgA1-439 plus truncated fragments lacking part or the entire C-terminal region) were assessed by specific ELISAs.RESULTS: Plasma levels of VS-1 and total-CgA correlated with carotid artery maximum stenosis (r=0.349, p=0.001 and r=0.256, p=0.021, respectively). Stepwise multiple regression analysis indicated that VS-1 was a significant predictor of maximum stenosis after adjustment for age, gender, and conventional risk factors for atherosclerosis (regression coefficient=12.42, SE=4.84, p=0.012). In addition, logistic regression analysis indicated that relatively high levels of full-length CgA, but not total-CgA, predict the presence of hypoechoic, lipid-rich plaques (OR=1.47; 95% CI: 1.19-1.81, p=0.0003).CONCLUSION: VS-1 is independently associated with carotid artery maximum stenosis. Furthermore, full-length CgA is an independent indicator of hypoechoic plaques, likely reflecting initial stages of atherosclerosis. Given the known capability of CgA and VS-1 to regulate vascular function and angiogenesis these polypeptides might play a role in the regulation of atherosclerosis pathophysiology.

KW - Journal Article

U2 - 10.1016/j.ijcard.2017.02.019

DO - 10.1016/j.ijcard.2017.02.019

M3 - Article

C2 - 28190616

VL - 236

SP - 438

EP - 443

JO - International Journal of Cardiology

JF - International Journal of Cardiology

SN - 0167-5273

ER -