Plasma levels of vasostatin-1, a chromogranin A fragment, are associated with carotid artery maximum stenosis: A pilot study

T Bachetti, A Ferrari Bardile, TL Aloi, B Colombo, E Assi, G Savino, A Vercelli, R Colombo, A Corti

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Abstract

Background Chromogranin A (CgA), a circulating protein released by the neuroendocrine system, can regulate vascular physiology and angiogenesis. Full-length CgA (CgA1–439) and its fragment CgA1–76 (called vasostatin-1, VS-1) preserve the physiological integrity of the endothelial barrier function and are antiangiogenic, whereas CgA1–373 is proangiogenic. We investigated whether these polypeptides are altered in patients with various degrees of carotid artery atherosclerosis. Methods We studied 81 patients with carotid artery atherosclerosis, asymptomatic for cerebrovascular diseases. Carotid arteries were examined by Doppler ultrasound and plaque characteristics were recorded. Plasma levels of CgA1–439, VS-1, CgA1–373, and total-CgA (CgA1–439 plus truncated fragments lacking part or the entire C-terminal region) were assessed by specific ELISAs. Results Plasma levels of VS-1 and total-CgA correlated with carotid artery maximum stenosis (r = 0.349, p = 0.001 and r = 0.256, p = 0.021, respectively). Stepwise multiple regression analysis indicated that VS-1 was a significant predictor of maximum stenosis after adjustment for age, gender, and conventional risk factors for atherosclerosis (regression coefficient = 12.42, SE = 4.84, p = 0.012). In addition, logistic regression analysis indicated that relatively high levels of full-length CgA, but not total-CgA, predict the presence of hypoechoic, lipid-rich plaques (OR = 1.47; 95% CI: 1.19–1.81, p = 0.0003). Conclusion VS-1 is independently associated with carotid artery maximum stenosis. Furthermore, full-length CgA is an independent indicator of hypoechoic plaques, likely reflecting initial stages of atherosclerosis. Given the known capability of CgA and VS-1 to regulate vascular function and angiogenesis these polypeptides might play a role in the regulation of atherosclerosis pathophysiology. © 2017 Elsevier B.V.
Original languageEnglish
Pages (from-to)438-443
Number of pages6
JournalInternational Journal of Cardiology
Volume236
Issue number5
DOIs
Publication statusPublished - 2017

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Chromogranin A
Carotid Stenosis
Carotid Arteries
Atherosclerosis
Carotid Artery Diseases
Blood Vessels
Regression Analysis
Cerebrovascular Disorders
Asymptomatic Diseases
Doppler Ultrasonography
Peptides
Neurosecretory Systems
vasostatin I
Pathologic Constriction
Logistic Models
Enzyme-Linked Immunosorbent Assay
Lipids

Cite this

@article{6a2ae54a5f504438a0f9c27c9dd01fd9,
title = "Plasma levels of vasostatin-1, a chromogranin A fragment, are associated with carotid artery maximum stenosis: A pilot study",
abstract = "Background Chromogranin A (CgA), a circulating protein released by the neuroendocrine system, can regulate vascular physiology and angiogenesis. Full-length CgA (CgA1–439) and its fragment CgA1–76 (called vasostatin-1, VS-1) preserve the physiological integrity of the endothelial barrier function and are antiangiogenic, whereas CgA1–373 is proangiogenic. We investigated whether these polypeptides are altered in patients with various degrees of carotid artery atherosclerosis. Methods We studied 81 patients with carotid artery atherosclerosis, asymptomatic for cerebrovascular diseases. Carotid arteries were examined by Doppler ultrasound and plaque characteristics were recorded. Plasma levels of CgA1–439, VS-1, CgA1–373, and total-CgA (CgA1–439 plus truncated fragments lacking part or the entire C-terminal region) were assessed by specific ELISAs. Results Plasma levels of VS-1 and total-CgA correlated with carotid artery maximum stenosis (r = 0.349, p = 0.001 and r = 0.256, p = 0.021, respectively). Stepwise multiple regression analysis indicated that VS-1 was a significant predictor of maximum stenosis after adjustment for age, gender, and conventional risk factors for atherosclerosis (regression coefficient = 12.42, SE = 4.84, p = 0.012). In addition, logistic regression analysis indicated that relatively high levels of full-length CgA, but not total-CgA, predict the presence of hypoechoic, lipid-rich plaques (OR = 1.47; 95{\%} CI: 1.19–1.81, p = 0.0003). Conclusion VS-1 is independently associated with carotid artery maximum stenosis. Furthermore, full-length CgA is an independent indicator of hypoechoic plaques, likely reflecting initial stages of atherosclerosis. Given the known capability of CgA and VS-1 to regulate vascular function and angiogenesis these polypeptides might play a role in the regulation of atherosclerosis pathophysiology. {\circledC} 2017 Elsevier B.V.",
author = "T Bachetti and {Ferrari Bardile}, A and TL Aloi and B Colombo and E Assi and G Savino and A Vercelli and R Colombo and A Corti",
year = "2017",
doi = "10.1016/j.ijcard.2017.02.019",
language = "English",
volume = "236",
pages = "438--443",
journal = "International Journal of Cardiology",
issn = "0167-5273",
publisher = "Elsevier Ireland Ltd",
number = "5",

}

TY - JOUR

T1 - Plasma levels of vasostatin-1, a chromogranin A fragment, are associated with carotid artery maximum stenosis: A pilot study

AU - Bachetti, T

AU - Ferrari Bardile, A

AU - Aloi, TL

AU - Colombo, B

AU - Assi, E

AU - Savino, G

AU - Vercelli, A

AU - Colombo, R

AU - Corti, A

PY - 2017

Y1 - 2017

N2 - Background Chromogranin A (CgA), a circulating protein released by the neuroendocrine system, can regulate vascular physiology and angiogenesis. Full-length CgA (CgA1–439) and its fragment CgA1–76 (called vasostatin-1, VS-1) preserve the physiological integrity of the endothelial barrier function and are antiangiogenic, whereas CgA1–373 is proangiogenic. We investigated whether these polypeptides are altered in patients with various degrees of carotid artery atherosclerosis. Methods We studied 81 patients with carotid artery atherosclerosis, asymptomatic for cerebrovascular diseases. Carotid arteries were examined by Doppler ultrasound and plaque characteristics were recorded. Plasma levels of CgA1–439, VS-1, CgA1–373, and total-CgA (CgA1–439 plus truncated fragments lacking part or the entire C-terminal region) were assessed by specific ELISAs. Results Plasma levels of VS-1 and total-CgA correlated with carotid artery maximum stenosis (r = 0.349, p = 0.001 and r = 0.256, p = 0.021, respectively). Stepwise multiple regression analysis indicated that VS-1 was a significant predictor of maximum stenosis after adjustment for age, gender, and conventional risk factors for atherosclerosis (regression coefficient = 12.42, SE = 4.84, p = 0.012). In addition, logistic regression analysis indicated that relatively high levels of full-length CgA, but not total-CgA, predict the presence of hypoechoic, lipid-rich plaques (OR = 1.47; 95% CI: 1.19–1.81, p = 0.0003). Conclusion VS-1 is independently associated with carotid artery maximum stenosis. Furthermore, full-length CgA is an independent indicator of hypoechoic plaques, likely reflecting initial stages of atherosclerosis. Given the known capability of CgA and VS-1 to regulate vascular function and angiogenesis these polypeptides might play a role in the regulation of atherosclerosis pathophysiology. © 2017 Elsevier B.V.

AB - Background Chromogranin A (CgA), a circulating protein released by the neuroendocrine system, can regulate vascular physiology and angiogenesis. Full-length CgA (CgA1–439) and its fragment CgA1–76 (called vasostatin-1, VS-1) preserve the physiological integrity of the endothelial barrier function and are antiangiogenic, whereas CgA1–373 is proangiogenic. We investigated whether these polypeptides are altered in patients with various degrees of carotid artery atherosclerosis. Methods We studied 81 patients with carotid artery atherosclerosis, asymptomatic for cerebrovascular diseases. Carotid arteries were examined by Doppler ultrasound and plaque characteristics were recorded. Plasma levels of CgA1–439, VS-1, CgA1–373, and total-CgA (CgA1–439 plus truncated fragments lacking part or the entire C-terminal region) were assessed by specific ELISAs. Results Plasma levels of VS-1 and total-CgA correlated with carotid artery maximum stenosis (r = 0.349, p = 0.001 and r = 0.256, p = 0.021, respectively). Stepwise multiple regression analysis indicated that VS-1 was a significant predictor of maximum stenosis after adjustment for age, gender, and conventional risk factors for atherosclerosis (regression coefficient = 12.42, SE = 4.84, p = 0.012). In addition, logistic regression analysis indicated that relatively high levels of full-length CgA, but not total-CgA, predict the presence of hypoechoic, lipid-rich plaques (OR = 1.47; 95% CI: 1.19–1.81, p = 0.0003). Conclusion VS-1 is independently associated with carotid artery maximum stenosis. Furthermore, full-length CgA is an independent indicator of hypoechoic plaques, likely reflecting initial stages of atherosclerosis. Given the known capability of CgA and VS-1 to regulate vascular function and angiogenesis these polypeptides might play a role in the regulation of atherosclerosis pathophysiology. © 2017 Elsevier B.V.

U2 - 10.1016/j.ijcard.2017.02.019

DO - 10.1016/j.ijcard.2017.02.019

M3 - Article

VL - 236

SP - 438

EP - 443

JO - International Journal of Cardiology

JF - International Journal of Cardiology

SN - 0167-5273

IS - 5

ER -