Plasma lipoproteins and cholesterol metabolism in spontaneously hyperlipemic rats

Simona Fantappiè, Maurizio Crestani, Enrica Bosisio, Giovanni Galli, Franco M. Maggi, Alberto Corsini, Alberico L. Catapanoff

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of this study was to characterize the plasma lipoprotein pattern and some aspects of cholesterol metabolism in a line of hyperlipemic male rats. Plasma cholesterol and triglycerides were increased about 3-fold as compared to control animals (238 vs. 75 and 185 vs. 59 mg/dl respectively). The plasma lipoprotein distribution and the chemical composition of the isolated lipoprotein unaffected. Plasma triglyceri production rate was increased (40%, P <0.01) and post-heparin lipoprotein lipase activity in plasma decreased (-28%, P <0.01) in the hyperlipemic rat. The activity of 3 enzymes involved in cholesterol metabolism (HMG-CoA reductase, cholesterol 7α-hydroxylase, and acyl-CoA cholesterol-acyltransferase) did not differ from control values. 3H20 incorporation into digitonin-precipitable sterols, however, was significantly higher than in controls. This finding was due, in part, to an increased liver weight in the hyperlipemic animals. Furthermore kinetic data using 125I-LDL showed that the fractional catabolic rate of lipoprotein was within the normal range, while the synthetic rate of LDL protein was increased (0.67 vs. 0.3 mg/kg/h, P <0.01) in the hyperlipemic rat. These observations suggest that multiple metabolic defects underline the hyperlipemia observed in this animal model.

Original languageEnglish
Pages (from-to)163-171
Number of pages9
JournalAtherosclerosis
Volume76
Issue number2-3
DOIs
Publication statusPublished - 1989

Keywords

  • ACAT
  • Cholesterol 7α-hydroxylase
  • Hepatic lipase
  • HMG-CoA reductase
  • Lipoprotein lipase
  • Lipoprotein receptor

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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