Plasma membrane-associated glycohydrolases activation by extracellular acidification due to proton exchangers

Massimo Aureli, Nicoletta Loberto, Rosaria Bassi, Anita Ferraretto, Silvia Perego, Patrizia Lanteri, Vanna Chigorno, Sandro Sonnino, Alessandro Prinetti

Research output: Contribution to journalArticlepeer-review

Abstract

In this paper, we show that the pH optimum for the plasma membrane (PM)-associated activity of four glycohydrolases (conduritol B epoxide sensitive β-glucosidase, β-glucosidase GBA2, b-hexosaminidase and bgalactosidase) measured on intact cells is acidic. Moreover, we show that drugs able to modify the efflux of protons across the PM, thus locally affecting the extracellular proton concentration close to the PM, are able to modulate the activities of these enzymes. These data strongly suggest that pH-dependent modulation of PM-associated glycohydrolases activities could be an effective way to locally modulate the cell surface glycoconjugate composition.

Original languageEnglish
Pages (from-to)1296-1307
Number of pages12
JournalNeurochemical Research
Volume37
Issue number6
DOIs
Publication statusPublished - Jun 2012

Keywords

  • Glycoconjugates
  • Glycohydrolases
  • Glycolipids
  • Glycoproteins
  • Optimal pH
  • Plasma membranes
  • Proton modulators

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Biochemistry

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