Plasma membrane-associated glycohydrolases along differentiation of murine neural stem cells

Massimo Aureli, Angela Gritti, Rosaria Bassi, Nicoletta Loberto, Alessandra Ricca, Vanna Chigorno, Alessandro Prinetti, Sandro Sonnino

Research output: Contribution to journalArticlepeer-review

Abstract

The activities of plasma membrane associated sialidase Neu3, total β-glucosidase, CBE-sensitive β-glucosidase, non-lysosomal β-glucosyl ceramidase GBA2, β-galactosidase, β-hexosaminidase and sphingomyelinase were determined at three different stages of differentiation of murine neural stem cell cultures, corresponding to precursors, commited progenitors, and differentiated cells. Cell immunostaining for specific markers of the differentiation process, performed after 7 days in culture in presence of differentiating agents, clearly showed the presence of oligodendrocytes, astrocytes and neurons. Glial cells were the most abundant. Sialidase Neu3 after a decrease from progenitors to precursors, showed an increase parallel to the differentiation process. All the other glycosidases increased their activity along differentiation. The activity of CBE-sensitive β-glucosidase and GBA2 were very similar at the precursor stage, but CBE-sensitive β-glucosidase increased 7 times while GBA2 only two in the differentiated cells. In addition, we analysed also sphingomyelinase as enzyme specifically associated to sphingolipids. The activity of this enzyme increased from precursors to differentiated cells.

Original languageEnglish
Pages (from-to)1344-1354
Number of pages11
JournalNeurochemical Research
Volume37
Issue number6
DOIs
Publication statusPublished - Jun 2012

Keywords

  • Glycoconjugates
  • Hydrolases
  • Plasma membrane
  • Sphingolipids
  • Stem cells

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Biochemistry

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