Plasma membrane hyperpolarization and [Ca2+]i increase induced by fibroblast growth factor in NIH-3T3 fibroblasts

Resemblance to early signals generated by platelet-derived growth factor

Atanasio Pandiella, Michele Magni, Jacopo Meldolesi

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The effects of different substances on [Ca2+]i and membrane potential (measured by fura-2 and bis-oxonol fluorescence techniques, respectively) were studied in wild-type and NIH-3T3 fibroblasts transfected with the cDNA encoding the human epidermal growth factor receptor. Application of partially purified PDGF or FGF induced, after a lag (0.5-1 min), a [Ca2+]i increase composed by an initial, slow peak, sustained primarily by intracellular Ca2+ release followed by a plateau, sustained by Ca2+ influx from the medium. The [Ca2+]i changes were paralleled by plasma membrane hyperpolarization mainly due to the activation of a K+ efflux, since raising the extracellular K+ concentration progressively reversed the effect of both growth factors. These responses were much slower than those induced by other agents (bradykinin, extracellular ATP, and EGF). The close resemblance between PDGF- and FGF-induced early signals (time-course and insensitivity to phorbol esters) suggests similar transmembrane signalling mechanisms at the cognate receptor.

Original languageEnglish
Pages (from-to)1325-1331
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume163
Issue number3
DOIs
Publication statusPublished - Sep 29 1989

Fingerprint

Fibroblast Growth Factors
Fura-2
Platelet-Derived Growth Factor
Bradykinin
Phorbol Esters
Fibroblasts
Cell membranes
Epidermal Growth Factor Receptor
Epidermal Growth Factor
Membrane Potentials
Intercellular Signaling Peptides and Proteins
Complementary DNA
Adenosine Triphosphate
Fluorescence
Chemical activation
Cell Membrane
Membranes
bis(1,3-diethylthiobarbiturate)trimethineoxonol

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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title = "Plasma membrane hyperpolarization and [Ca2+]i increase induced by fibroblast growth factor in NIH-3T3 fibroblasts: Resemblance to early signals generated by platelet-derived growth factor",
abstract = "The effects of different substances on [Ca2+]i and membrane potential (measured by fura-2 and bis-oxonol fluorescence techniques, respectively) were studied in wild-type and NIH-3T3 fibroblasts transfected with the cDNA encoding the human epidermal growth factor receptor. Application of partially purified PDGF or FGF induced, after a lag (0.5-1 min), a [Ca2+]i increase composed by an initial, slow peak, sustained primarily by intracellular Ca2+ release followed by a plateau, sustained by Ca2+ influx from the medium. The [Ca2+]i changes were paralleled by plasma membrane hyperpolarization mainly due to the activation of a K+ efflux, since raising the extracellular K+ concentration progressively reversed the effect of both growth factors. These responses were much slower than those induced by other agents (bradykinin, extracellular ATP, and EGF). The close resemblance between PDGF- and FGF-induced early signals (time-course and insensitivity to phorbol esters) suggests similar transmembrane signalling mechanisms at the cognate receptor.",
author = "Atanasio Pandiella and Michele Magni and Jacopo Meldolesi",
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