Plasma mitomycin C concentrations determined by HPLC coupled to solid- phase extraction

Rita Paroni, Cinzia Arcelloni, Elena De Vecchi, Isabella Fermo, Davide Mauri, Renzo Colombo

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of this study was to set up a method for quantification of plasma mitomycin C (MMC) concentrations during intravesical chemotherapy delivered in the presence of local bladder hyperthermia (HT). In comparison with existing methods, this assay, characterized by relative simplicity and efficiency, resulted in the facilitation of performance with nondedicated instrumentation or nonspecialized staff. Purification from plasma matrix was carried out by solid-phase extraction under vacuum. The purified drug was then collected directly into the vials of the HPLC autosampler. Chromatographic analysis was performed on a reversed-phase C18 column with water:acetonitrile (85:15 by vol) as the mobile phase and the UV detector set at 365 nm. The use of porfiromycin as internal standard provided a method with good within-day precision (CV 6.0% at 5 μg/L, n = 6), linearity (0.5- 50 μg/L), and specificity. The lower limit of detection (≤0.5 μg/L) proved to be suitable for plasma pharmacokinetics monitoring in two tested patients treated with MMC+HT for superficial bladder cancer.

Original languageEnglish
Pages (from-to)615-618
Number of pages4
JournalClinical Chemistry
Volume43
Issue number4
Publication statusPublished - 1997

Keywords

  • bladder hyperthermia
  • intravesical chemotherapy
  • pharmacokinetics
  • plasma assay
  • porfiromycin

ASJC Scopus subject areas

  • Clinical Biochemistry

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