Plasma ochratoxin A levels, food consumption, and risk biomarkers of a representative sample of men and women from the Molise region in Italy

Romina Di Giuseppe, Terenzio Bertuzzi, Filippo Rossi, Silvia Rastelli, Annalisa Mulazzi, Jessica Capraro, Amalia De Curtis, Licia Iacoviello, Amedeo Pietri

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: Ochratoxin A (OTA) is a mycotoxin present in food that can be found in human blood, due to its long half-life. Plasma OTA detection represents a good parameter for evaluating the exposure at the population level. Purpose: The relation between plasma OTA levels, dietary habits, and specific disease risk biomarkers (body mass index (BMI), C-reactive protein (CRP), and cardiovascular risk score) was investigated. Methods: The study involved 327 subjects (150 men and 177 women) aged between 38 and 48 years. Food consumption was evaluated by means of the EPIC questionnaire; plasma OTA was measured by HPLC; CRP was determined in fresh serum samples by a latex particleenhanced immunoturbidimetric assay. Results: OTA was detected in 99.1% of plasma samples (LOD 25 ng/L); the mean ± SD value was 0.229 ± 0.238 ng/mL. However, only 5.2% of samples exceeded 500 ng/L, considered the threshold for a possible pathogenic activity. The estimated mean daily dietary intake of OTA resulted 0.452 ± 0.468 ng/kg body weight (bw)/day, markedly lower than the tolerable daily intake set by EFSA (17.1 ng/kg bw/day). Processed and mutton/lamb meat were found to contribute most to plasma OTA variance. Nevertheless, cereals, wine, beer, and jam/honey consumption correlated positively with OTA levels. Plasma OTA showed a significant positive association with CRP and cardiovascular risk score (β = 0.20 ± 0.08; P = 0.015 and β = 0.25 ± 0.08; P = 0.001, respectively); however, the association was present in men but not in women. Conclusions: Even if the hypothesis of a possible hepatic toxicity of OTA in humans is yet to be verified, the positive association between plasma OTA and CRP may indicate a possible role of OTA in inflammation status and consequently in the genesis of cardiovascular diseases and cancer.

Original languageEnglish
Pages (from-to)851-860
Number of pages10
JournalEuropean Journal of Nutrition
Volume51
Issue number7
DOIs
Publication statusPublished - Oct 2012

Fingerprint

Italy
Biomarkers
Food
C-Reactive Protein
ochratoxin A
Body Weight
No-Observed-Adverse-Effect Level
Honey
Mycotoxins
Latex
Feeding Behavior
Wine
Meat
Half-Life
Body Mass Index
Cardiovascular Diseases
High Pressure Liquid Chromatography
Inflammation
Liver

Keywords

  • C-reactive protein
  • Cancer
  • Cardiovascular disease
  • Ochratoxin A

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Plasma ochratoxin A levels, food consumption, and risk biomarkers of a representative sample of men and women from the Molise region in Italy. / Di Giuseppe, Romina; Bertuzzi, Terenzio; Rossi, Filippo; Rastelli, Silvia; Mulazzi, Annalisa; Capraro, Jessica; De Curtis, Amalia; Iacoviello, Licia; Pietri, Amedeo.

In: European Journal of Nutrition, Vol. 51, No. 7, 10.2012, p. 851-860.

Research output: Contribution to journalArticle

Di Giuseppe, Romina ; Bertuzzi, Terenzio ; Rossi, Filippo ; Rastelli, Silvia ; Mulazzi, Annalisa ; Capraro, Jessica ; De Curtis, Amalia ; Iacoviello, Licia ; Pietri, Amedeo. / Plasma ochratoxin A levels, food consumption, and risk biomarkers of a representative sample of men and women from the Molise region in Italy. In: European Journal of Nutrition. 2012 ; Vol. 51, No. 7. pp. 851-860.
@article{e5dcef2e8c094306821b1cb3c253fb77,
title = "Plasma ochratoxin A levels, food consumption, and risk biomarkers of a representative sample of men and women from the Molise region in Italy",
abstract = "Background: Ochratoxin A (OTA) is a mycotoxin present in food that can be found in human blood, due to its long half-life. Plasma OTA detection represents a good parameter for evaluating the exposure at the population level. Purpose: The relation between plasma OTA levels, dietary habits, and specific disease risk biomarkers (body mass index (BMI), C-reactive protein (CRP), and cardiovascular risk score) was investigated. Methods: The study involved 327 subjects (150 men and 177 women) aged between 38 and 48 years. Food consumption was evaluated by means of the EPIC questionnaire; plasma OTA was measured by HPLC; CRP was determined in fresh serum samples by a latex particleenhanced immunoturbidimetric assay. Results: OTA was detected in 99.1{\%} of plasma samples (LOD 25 ng/L); the mean ± SD value was 0.229 ± 0.238 ng/mL. However, only 5.2{\%} of samples exceeded 500 ng/L, considered the threshold for a possible pathogenic activity. The estimated mean daily dietary intake of OTA resulted 0.452 ± 0.468 ng/kg body weight (bw)/day, markedly lower than the tolerable daily intake set by EFSA (17.1 ng/kg bw/day). Processed and mutton/lamb meat were found to contribute most to plasma OTA variance. Nevertheless, cereals, wine, beer, and jam/honey consumption correlated positively with OTA levels. Plasma OTA showed a significant positive association with CRP and cardiovascular risk score (β = 0.20 ± 0.08; P = 0.015 and β = 0.25 ± 0.08; P = 0.001, respectively); however, the association was present in men but not in women. Conclusions: Even if the hypothesis of a possible hepatic toxicity of OTA in humans is yet to be verified, the positive association between plasma OTA and CRP may indicate a possible role of OTA in inflammation status and consequently in the genesis of cardiovascular diseases and cancer.",
keywords = "C-reactive protein, Cancer, Cardiovascular disease, Ochratoxin A",
author = "{Di Giuseppe}, Romina and Terenzio Bertuzzi and Filippo Rossi and Silvia Rastelli and Annalisa Mulazzi and Jessica Capraro and {De Curtis}, Amalia and Licia Iacoviello and Amedeo Pietri",
year = "2012",
month = "10",
doi = "10.1007/s00394-011-0265-5",
language = "English",
volume = "51",
pages = "851--860",
journal = "European Journal of Nutrition",
issn = "1436-6207",
publisher = "D. Steinkopff-Verlag",
number = "7",

}

TY - JOUR

T1 - Plasma ochratoxin A levels, food consumption, and risk biomarkers of a representative sample of men and women from the Molise region in Italy

AU - Di Giuseppe, Romina

AU - Bertuzzi, Terenzio

AU - Rossi, Filippo

AU - Rastelli, Silvia

AU - Mulazzi, Annalisa

AU - Capraro, Jessica

AU - De Curtis, Amalia

AU - Iacoviello, Licia

AU - Pietri, Amedeo

PY - 2012/10

Y1 - 2012/10

N2 - Background: Ochratoxin A (OTA) is a mycotoxin present in food that can be found in human blood, due to its long half-life. Plasma OTA detection represents a good parameter for evaluating the exposure at the population level. Purpose: The relation between plasma OTA levels, dietary habits, and specific disease risk biomarkers (body mass index (BMI), C-reactive protein (CRP), and cardiovascular risk score) was investigated. Methods: The study involved 327 subjects (150 men and 177 women) aged between 38 and 48 years. Food consumption was evaluated by means of the EPIC questionnaire; plasma OTA was measured by HPLC; CRP was determined in fresh serum samples by a latex particleenhanced immunoturbidimetric assay. Results: OTA was detected in 99.1% of plasma samples (LOD 25 ng/L); the mean ± SD value was 0.229 ± 0.238 ng/mL. However, only 5.2% of samples exceeded 500 ng/L, considered the threshold for a possible pathogenic activity. The estimated mean daily dietary intake of OTA resulted 0.452 ± 0.468 ng/kg body weight (bw)/day, markedly lower than the tolerable daily intake set by EFSA (17.1 ng/kg bw/day). Processed and mutton/lamb meat were found to contribute most to plasma OTA variance. Nevertheless, cereals, wine, beer, and jam/honey consumption correlated positively with OTA levels. Plasma OTA showed a significant positive association with CRP and cardiovascular risk score (β = 0.20 ± 0.08; P = 0.015 and β = 0.25 ± 0.08; P = 0.001, respectively); however, the association was present in men but not in women. Conclusions: Even if the hypothesis of a possible hepatic toxicity of OTA in humans is yet to be verified, the positive association between plasma OTA and CRP may indicate a possible role of OTA in inflammation status and consequently in the genesis of cardiovascular diseases and cancer.

AB - Background: Ochratoxin A (OTA) is a mycotoxin present in food that can be found in human blood, due to its long half-life. Plasma OTA detection represents a good parameter for evaluating the exposure at the population level. Purpose: The relation between plasma OTA levels, dietary habits, and specific disease risk biomarkers (body mass index (BMI), C-reactive protein (CRP), and cardiovascular risk score) was investigated. Methods: The study involved 327 subjects (150 men and 177 women) aged between 38 and 48 years. Food consumption was evaluated by means of the EPIC questionnaire; plasma OTA was measured by HPLC; CRP was determined in fresh serum samples by a latex particleenhanced immunoturbidimetric assay. Results: OTA was detected in 99.1% of plasma samples (LOD 25 ng/L); the mean ± SD value was 0.229 ± 0.238 ng/mL. However, only 5.2% of samples exceeded 500 ng/L, considered the threshold for a possible pathogenic activity. The estimated mean daily dietary intake of OTA resulted 0.452 ± 0.468 ng/kg body weight (bw)/day, markedly lower than the tolerable daily intake set by EFSA (17.1 ng/kg bw/day). Processed and mutton/lamb meat were found to contribute most to plasma OTA variance. Nevertheless, cereals, wine, beer, and jam/honey consumption correlated positively with OTA levels. Plasma OTA showed a significant positive association with CRP and cardiovascular risk score (β = 0.20 ± 0.08; P = 0.015 and β = 0.25 ± 0.08; P = 0.001, respectively); however, the association was present in men but not in women. Conclusions: Even if the hypothesis of a possible hepatic toxicity of OTA in humans is yet to be verified, the positive association between plasma OTA and CRP may indicate a possible role of OTA in inflammation status and consequently in the genesis of cardiovascular diseases and cancer.

KW - C-reactive protein

KW - Cancer

KW - Cardiovascular disease

KW - Ochratoxin A

UR - http://www.scopus.com/inward/record.url?scp=84870242470&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84870242470&partnerID=8YFLogxK

U2 - 10.1007/s00394-011-0265-5

DO - 10.1007/s00394-011-0265-5

M3 - Article

C2 - 22038465

AN - SCOPUS:84870242470

VL - 51

SP - 851

EP - 860

JO - European Journal of Nutrition

JF - European Journal of Nutrition

SN - 1436-6207

IS - 7

ER -