Plasma sRAGE and N-(carboxymethyl) lysine in patients with CHF and/or COPD

Piera Boschetto, Ilaria Campo, Mariarita Stendardo, Enrico Casimirri, Carmine Tinelli, Marina Gorrini, Claudio Ceconi, Alessandro Fucili, Alfredo Potena, Alberto Papi, Licia Ballerin, Leonardo Fabbri, Maurizio Luisetti

Research output: Contribution to journalArticle

Abstract

Background: Knowledge of the role of the receptor for advanced glycation end products (RAGE), particularly its soluble form (sRAGE), and of its advanced glycation end product (AGE) ligand, N-(carboxymethyl)lysine adducts (CML), is limited in chronic heart failure (CHF) and in chronic obstructive pulmonary disease (COPD). We evaluated whether the AGE/RAGE system is activated in stable CHF and COPD, and whether plasma sRAGE and CML levels are affected by clinical and functional parameters. Materials and methods: We measured plasma levels of sRAGE and CML using a sandwich enzyme-linked immunosorbent assay (ELISA) in 143 subjects, aged ≥ 65 years, divided into five groups: 58 with CHF, 23 with COPD, 27 with CHF+COPD and 35 controls (17 healthy smokers and 18 healthy nonsmokers). Individuals with diabetes were excluded from the study. Results: Plasma levels of sRAGE and CML were higher in CHF patients than in controls [sRAGE: 0·48 (0·37-0·83) vs. 0·42 (0·29-0·52) ng/mL, P = 0·01; CML: 1·95 (1·58-2·38) vs. 1·68 (1·43-2·00) ng/mL, P = 0·01]. By contrast, sRAGE and CML were not different between both COPD and CHF+COPD patients and controls (P > 0·05). N-terminal pro-brain natriuretic peptide (Nt-pro BNP) correlated with sRAGE, but not with CML, in the patient groups: CHF (r = 0·43, P <0·001), COPD (r = 0·77, P <0·0001) and CHF/COPD (r = 0·43, P = 0·003). Conclusions: Plasma levels of sRAGE and CML are increased in CHF, but not in COPD patients. The robust association between NT-pro BNP, a diagnostic and prognostic marker in CHF, and sRAGE concentrations might suggest a possible BNP pathway of amplification of inflammation via the AGE/RAGE system.

Original languageEnglish
Pages (from-to)562-569
Number of pages8
JournalEuropean Journal of Clinical Investigation
Volume43
Issue number6
DOIs
Publication statusPublished - Jun 2013

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Keywords

  • Advanced glycation end products
  • Chronic heart failure
  • Chronic obstructive pulmonary disease
  • Inflammation
  • N-terminal pro-brain natriuretic peptide
  • Soluble receptor for advanced glycation end products

ASJC Scopus subject areas

  • Medicine(all)
  • Clinical Biochemistry
  • Biochemistry

Cite this

Boschetto, P., Campo, I., Stendardo, M., Casimirri, E., Tinelli, C., Gorrini, M., Ceconi, C., Fucili, A., Potena, A., Papi, A., Ballerin, L., Fabbri, L., & Luisetti, M. (2013). Plasma sRAGE and N-(carboxymethyl) lysine in patients with CHF and/or COPD. European Journal of Clinical Investigation, 43(6), 562-569. https://doi.org/10.1111/eci.12079