Plasma viremia and virus phenotype are correlates of disease progression in vertically human immunodeficiency virus type 1-infected children

Claudia Balotta, M. Chiara Colombo, Giuseppe Colucci, Alessandra Viganò, Chiara Riva, Laura Papagno, Michela Violin, Lina Crupi, Dorella Bricalli, Antonino Salvaggio, Mauro Moroni, Nicola Principi, Massimo Galli

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Objective. To analyze the relationships among HIV-1 plasma viremia, phenotype and CD4 T cell counts in vertically infected children. Methods. Plasma viremia was quantified in 37 vertically infected children at different stages of the disease by a standardized molecular assay. Virus isolation and non-syncytia-inducing or syncytia-inducing (SI) HIV-1 phenotype evaluation were performed in parallel. Results. HIV-1 RNA genomes were found to be significantly different in CDC clinical classes N, A, B and C (P = 0.0135) and in immunologic classes 1, 2 and 3 (P = 0.0110). None of the children in Class N or A harbored H1V-1 isolates with SI phenotype, whereas SI primary isolates were detected in 2 of 7 (29%) and 7 of 10 (70%) Class B and C children, respectively. Similarly SI variants were present in only 9 of 13 children in immunologic Class 3 (70%). When stratified according to the increasing severity of virologic status, the children showed a significant difference (P = 0.0458) in viral burden. Conclusions. Clinical symptoms, the most dramatic being reduction in the number of CD4 lymphocytes, and the highest plasma viremia levels were observed in the children in whom fast replicating, highly cytopathic SI variants were isolated. These data extend the virologic characterization of vertically HIV-1-infected children and suggest that both the plasma viremia levels and phenotype of primary isolates are viral correlates of disease progression in vertically infected children.

Original languageEnglish
Pages (from-to)205-211
Number of pages7
JournalPediatric Infectious Disease Journal
Volume16
Issue number2
DOIs
Publication statusPublished - Feb 1997

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Viremia
Disease Progression
HIV-1
Viruses
Phenotype
Giant Cells
Lymphocyte Count
Virus Diseases
Centers for Disease Control and Prevention (U.S.)
CD4 Lymphocyte Count
Viral Load
Genome
RNA
T-Lymphocytes

Keywords

  • CD4 T cells
  • human immunodeficiency virus type 1 plasma viremia
  • Human immunodeficiency virus type 1 vertical infection
  • isolation and phenotype

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Microbiology (medical)

Cite this

Plasma viremia and virus phenotype are correlates of disease progression in vertically human immunodeficiency virus type 1-infected children. / Balotta, Claudia; Colombo, M. Chiara; Colucci, Giuseppe; Viganò, Alessandra; Riva, Chiara; Papagno, Laura; Violin, Michela; Crupi, Lina; Bricalli, Dorella; Salvaggio, Antonino; Moroni, Mauro; Principi, Nicola; Galli, Massimo.

In: Pediatric Infectious Disease Journal, Vol. 16, No. 2, 02.1997, p. 205-211.

Research output: Contribution to journalArticle

Balotta, C, Colombo, MC, Colucci, G, Viganò, A, Riva, C, Papagno, L, Violin, M, Crupi, L, Bricalli, D, Salvaggio, A, Moroni, M, Principi, N & Galli, M 1997, 'Plasma viremia and virus phenotype are correlates of disease progression in vertically human immunodeficiency virus type 1-infected children', Pediatric Infectious Disease Journal, vol. 16, no. 2, pp. 205-211. https://doi.org/10.1097/00006454-199702000-00008
Balotta, Claudia ; Colombo, M. Chiara ; Colucci, Giuseppe ; Viganò, Alessandra ; Riva, Chiara ; Papagno, Laura ; Violin, Michela ; Crupi, Lina ; Bricalli, Dorella ; Salvaggio, Antonino ; Moroni, Mauro ; Principi, Nicola ; Galli, Massimo. / Plasma viremia and virus phenotype are correlates of disease progression in vertically human immunodeficiency virus type 1-infected children. In: Pediatric Infectious Disease Journal. 1997 ; Vol. 16, No. 2. pp. 205-211.
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abstract = "Objective. To analyze the relationships among HIV-1 plasma viremia, phenotype and CD4 T cell counts in vertically infected children. Methods. Plasma viremia was quantified in 37 vertically infected children at different stages of the disease by a standardized molecular assay. Virus isolation and non-syncytia-inducing or syncytia-inducing (SI) HIV-1 phenotype evaluation were performed in parallel. Results. HIV-1 RNA genomes were found to be significantly different in CDC clinical classes N, A, B and C (P = 0.0135) and in immunologic classes 1, 2 and 3 (P = 0.0110). None of the children in Class N or A harbored H1V-1 isolates with SI phenotype, whereas SI primary isolates were detected in 2 of 7 (29{\%}) and 7 of 10 (70{\%}) Class B and C children, respectively. Similarly SI variants were present in only 9 of 13 children in immunologic Class 3 (70{\%}). When stratified according to the increasing severity of virologic status, the children showed a significant difference (P = 0.0458) in viral burden. Conclusions. Clinical symptoms, the most dramatic being reduction in the number of CD4 lymphocytes, and the highest plasma viremia levels were observed in the children in whom fast replicating, highly cytopathic SI variants were isolated. These data extend the virologic characterization of vertically HIV-1-infected children and suggest that both the plasma viremia levels and phenotype of primary isolates are viral correlates of disease progression in vertically infected children.",
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AU - Viganò, Alessandra

AU - Riva, Chiara

AU - Papagno, Laura

AU - Violin, Michela

AU - Crupi, Lina

AU - Bricalli, Dorella

AU - Salvaggio, Antonino

AU - Moroni, Mauro

AU - Principi, Nicola

AU - Galli, Massimo

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