TY - JOUR
T1 - Plasma viremia and virus phenotype are correlates of disease progression in vertically human immunodeficiency virus type 1-infected children
AU - Balotta, Claudia
AU - Colombo, M. Chiara
AU - Colucci, Giuseppe
AU - Viganò, Alessandra
AU - Riva, Chiara
AU - Papagno, Laura
AU - Violin, Michela
AU - Crupi, Lina
AU - Bricalli, Dorella
AU - Salvaggio, Antonino
AU - Moroni, Mauro
AU - Principi, Nicola
AU - Galli, Massimo
PY - 1997/2
Y1 - 1997/2
N2 - Objective. To analyze the relationships among HIV-1 plasma viremia, phenotype and CD4 T cell counts in vertically infected children. Methods. Plasma viremia was quantified in 37 vertically infected children at different stages of the disease by a standardized molecular assay. Virus isolation and non-syncytia-inducing or syncytia-inducing (SI) HIV-1 phenotype evaluation were performed in parallel. Results. HIV-1 RNA genomes were found to be significantly different in CDC clinical classes N, A, B and C (P = 0.0135) and in immunologic classes 1, 2 and 3 (P = 0.0110). None of the children in Class N or A harbored H1V-1 isolates with SI phenotype, whereas SI primary isolates were detected in 2 of 7 (29%) and 7 of 10 (70%) Class B and C children, respectively. Similarly SI variants were present in only 9 of 13 children in immunologic Class 3 (70%). When stratified according to the increasing severity of virologic status, the children showed a significant difference (P = 0.0458) in viral burden. Conclusions. Clinical symptoms, the most dramatic being reduction in the number of CD4 lymphocytes, and the highest plasma viremia levels were observed in the children in whom fast replicating, highly cytopathic SI variants were isolated. These data extend the virologic characterization of vertically HIV-1-infected children and suggest that both the plasma viremia levels and phenotype of primary isolates are viral correlates of disease progression in vertically infected children.
AB - Objective. To analyze the relationships among HIV-1 plasma viremia, phenotype and CD4 T cell counts in vertically infected children. Methods. Plasma viremia was quantified in 37 vertically infected children at different stages of the disease by a standardized molecular assay. Virus isolation and non-syncytia-inducing or syncytia-inducing (SI) HIV-1 phenotype evaluation were performed in parallel. Results. HIV-1 RNA genomes were found to be significantly different in CDC clinical classes N, A, B and C (P = 0.0135) and in immunologic classes 1, 2 and 3 (P = 0.0110). None of the children in Class N or A harbored H1V-1 isolates with SI phenotype, whereas SI primary isolates were detected in 2 of 7 (29%) and 7 of 10 (70%) Class B and C children, respectively. Similarly SI variants were present in only 9 of 13 children in immunologic Class 3 (70%). When stratified according to the increasing severity of virologic status, the children showed a significant difference (P = 0.0458) in viral burden. Conclusions. Clinical symptoms, the most dramatic being reduction in the number of CD4 lymphocytes, and the highest plasma viremia levels were observed in the children in whom fast replicating, highly cytopathic SI variants were isolated. These data extend the virologic characterization of vertically HIV-1-infected children and suggest that both the plasma viremia levels and phenotype of primary isolates are viral correlates of disease progression in vertically infected children.
KW - CD4 T cells
KW - human immunodeficiency virus type 1 plasma viremia
KW - Human immunodeficiency virus type 1 vertical infection
KW - isolation and phenotype
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U2 - 10.1097/00006454-199702000-00008
DO - 10.1097/00006454-199702000-00008
M3 - Article
C2 - 9041602
AN - SCOPUS:0031048286
VL - 16
SP - 205
EP - 211
JO - Pediatric Infectious Disease Journal
JF - Pediatric Infectious Disease Journal
SN - 0891-3668
IS - 2
ER -