Abstract
The blaCMY -2/4-carrying IncB/O/K-like plasmids of seven Escherichia coli strains from poultry, poultry meat and human urine samples were examined using comparative analysis of whole plasmid sequences. The incompatibility group was determined by analysis of the incRNAI region and conjugation assays with strains containing the IncK and IncB/O reference plasmids. Strains were additionally characterized using MLST and MIC determination. The complete DNA sequences of all plasmids showed an average nucleotide identity of 91.3%. Plasmids were detected in E. coli sequence type (ST) 131, ST38, ST420, ST1431, ST1564 and belonged to a new plasmid variant (IncK2) within the IncK and IncB/O groups. Notably, one E. coli from poultry meat and one from human contained the same plasmid. The presence of a common recently recognized IncK2 plasmid in diverse E. coli from human urine isolates and poultry meat production suggests that the IncK2 plasmids originated from a common progenitor and have the capability to spread to genetically diverse E. coli in different reservoirs. This discovery is alarming and stresses the need of rapidly introducing strict hygiene measures throughout the food chain, limiting the spread of such plasmids in the human settings.
| Original language | English |
|---|---|
| Article number | 407 |
| Journal | Frontiers in Microbiology |
| Volume | 8 |
| Issue number | MAR |
| DOIs | |
| Publication status | Published - Mar 15 2017 |
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Keywords
- Animals
- Antibiotic resistance
- ESBL
- Food
- PAmpC
ASJC Scopus subject areas
- Microbiology
- Microbiology (medical)
Cite this
Plasmids carrying blaCMY -2/4 in Escherichia coli from poultry, poultry meat, and humans belong to a novel IncK subgroup designated IncK2. / Seiffert, Salome N.; Carattoli, Alessandra; Schwendener, Sybille; Collaud, Alexandra; Endimiani, Andrea; Perreten, Vincent.
In: Frontiers in Microbiology, Vol. 8, No. MAR, 407, 15.03.2017.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Plasmids carrying blaCMY -2/4 in Escherichia coli from poultry, poultry meat, and humans belong to a novel IncK subgroup designated IncK2
AU - Seiffert, Salome N.
AU - Carattoli, Alessandra
AU - Schwendener, Sybille
AU - Collaud, Alexandra
AU - Endimiani, Andrea
AU - Perreten, Vincent
PY - 2017/3/15
Y1 - 2017/3/15
N2 - The blaCMY -2/4-carrying IncB/O/K-like plasmids of seven Escherichia coli strains from poultry, poultry meat and human urine samples were examined using comparative analysis of whole plasmid sequences. The incompatibility group was determined by analysis of the incRNAI region and conjugation assays with strains containing the IncK and IncB/O reference plasmids. Strains were additionally characterized using MLST and MIC determination. The complete DNA sequences of all plasmids showed an average nucleotide identity of 91.3%. Plasmids were detected in E. coli sequence type (ST) 131, ST38, ST420, ST1431, ST1564 and belonged to a new plasmid variant (IncK2) within the IncK and IncB/O groups. Notably, one E. coli from poultry meat and one from human contained the same plasmid. The presence of a common recently recognized IncK2 plasmid in diverse E. coli from human urine isolates and poultry meat production suggests that the IncK2 plasmids originated from a common progenitor and have the capability to spread to genetically diverse E. coli in different reservoirs. This discovery is alarming and stresses the need of rapidly introducing strict hygiene measures throughout the food chain, limiting the spread of such plasmids in the human settings.
AB - The blaCMY -2/4-carrying IncB/O/K-like plasmids of seven Escherichia coli strains from poultry, poultry meat and human urine samples were examined using comparative analysis of whole plasmid sequences. The incompatibility group was determined by analysis of the incRNAI region and conjugation assays with strains containing the IncK and IncB/O reference plasmids. Strains were additionally characterized using MLST and MIC determination. The complete DNA sequences of all plasmids showed an average nucleotide identity of 91.3%. Plasmids were detected in E. coli sequence type (ST) 131, ST38, ST420, ST1431, ST1564 and belonged to a new plasmid variant (IncK2) within the IncK and IncB/O groups. Notably, one E. coli from poultry meat and one from human contained the same plasmid. The presence of a common recently recognized IncK2 plasmid in diverse E. coli from human urine isolates and poultry meat production suggests that the IncK2 plasmids originated from a common progenitor and have the capability to spread to genetically diverse E. coli in different reservoirs. This discovery is alarming and stresses the need of rapidly introducing strict hygiene measures throughout the food chain, limiting the spread of such plasmids in the human settings.
KW - Animals
KW - Antibiotic resistance
KW - ESBL
KW - Food
KW - PAmpC
UR - http://www.scopus.com/inward/record.url?scp=85016620209&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85016620209&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2017.00407
DO - 10.3389/fmicb.2017.00407
M3 - Article
AN - SCOPUS:85016620209
VL - 8
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
SN - 1664-302X
IS - MAR
M1 - 407
ER -