Plasmids carrying blaCMY -2/4 in Escherichia coli from poultry, poultry meat, and humans belong to a novel IncK subgroup designated IncK2

Salome N. Seiffert, Alessandra Carattoli, Sybille Schwendener, Alexandra Collaud, Andrea Endimiani, Vincent Perreten

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The blaCMY -2/4-carrying IncB/O/K-like plasmids of seven Escherichia coli strains from poultry, poultry meat and human urine samples were examined using comparative analysis of whole plasmid sequences. The incompatibility group was determined by analysis of the incRNAI region and conjugation assays with strains containing the IncK and IncB/O reference plasmids. Strains were additionally characterized using MLST and MIC determination. The complete DNA sequences of all plasmids showed an average nucleotide identity of 91.3%. Plasmids were detected in E. coli sequence type (ST) 131, ST38, ST420, ST1431, ST1564 and belonged to a new plasmid variant (IncK2) within the IncK and IncB/O groups. Notably, one E. coli from poultry meat and one from human contained the same plasmid. The presence of a common recently recognized IncK2 plasmid in diverse E. coli from human urine isolates and poultry meat production suggests that the IncK2 plasmids originated from a common progenitor and have the capability to spread to genetically diverse E. coli in different reservoirs. This discovery is alarming and stresses the need of rapidly introducing strict hygiene measures throughout the food chain, limiting the spread of such plasmids in the human settings.

Original languageEnglish
Article number407
JournalFrontiers in Microbiology
Volume8
Issue numberMAR
DOIs
Publication statusPublished - Mar 15 2017

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Poultry
Meat
Plasmids
Escherichia coli
Urine
Food Chain
Hygiene
Nucleotides

Keywords

  • Animals
  • Antibiotic resistance
  • ESBL
  • Food
  • PAmpC

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Plasmids carrying blaCMY -2/4 in Escherichia coli from poultry, poultry meat, and humans belong to a novel IncK subgroup designated IncK2. / Seiffert, Salome N.; Carattoli, Alessandra; Schwendener, Sybille; Collaud, Alexandra; Endimiani, Andrea; Perreten, Vincent.

In: Frontiers in Microbiology, Vol. 8, No. MAR, 407, 15.03.2017.

Research output: Contribution to journalArticle

Seiffert, Salome N. ; Carattoli, Alessandra ; Schwendener, Sybille ; Collaud, Alexandra ; Endimiani, Andrea ; Perreten, Vincent. / Plasmids carrying blaCMY -2/4 in Escherichia coli from poultry, poultry meat, and humans belong to a novel IncK subgroup designated IncK2. In: Frontiers in Microbiology. 2017 ; Vol. 8, No. MAR.
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