Plasmids pEMBLY: new single-stranded shuttle vectors for the recovery and analysis of yeast DNA sequences

C. Baldari, G. Cesareni

Research output: Contribution to journalArticlepeer-review

Abstract

We describe the construction and properties of pEMBLY plasmids. They belong to a new family of yeast shuttle vectors which are derived from plasmid vector pEMBL9 and offer the following improvement: (i) relatively small size; (ii) large number of cloning sites; (iii) screening for insert-containing plasmids on indicator plates; (iv) different combinations of genes which complement auxotrophic deficiencies and sequences that support DNA replication in Saccharomyces cerevisiae; and (v) ability to isolate the plasmid DNA in single-stranded (ss) form. The yeast S. cerevisiae can be efficiently transformed by these plasmids in both the ss and double-stranded (ds) forms. Finally, the presence of the phage f1 intergenic region allows one to obtain the cloned sequences in the ss form upon infection with the wild-type ss phage [Dotto et al., Virology 114 (1981) 463-473].

Original languageEnglish
Pages (from-to)27-32
Number of pages6
JournalGene
Volume35
Issue number1-2
DOIs
Publication statusPublished - 1985

Keywords

  • cloning sites
  • phage f1
  • Recombinant DNA
  • Saccharomyces cerevisiae
  • site-directed mutagenesis

ASJC Scopus subject areas

  • Genetics

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