Plasminogen assay in rabbit, rat and mouse plasma using the chromogenic substrate S-2251

L. Mussoni, E. Raczka, J. Chmielewska, M. B. Donati, Z. S. Latallo

Research output: Contribution to journalArticle

Abstract

An amidolytic assay has been developed for measuring plasminogen in rabbit, rat and mouse plasma using the synthetic substrate S-2251. The assay includes an acidification step to make plasminogen more readily activatable; the conditions required for optimal activation of plasminogen by urokinase were found to vary in the 3 animal species studied. Compared with human plasma, rat, mouse and rabbit plasma (in decreasing order) all had higher plasminogen activity. A close correlation was found between amidolytic and caseinolytic assay of plasminogen in all species studied. The method described may be of significant help in the study of the fibrinolytic system of laboratory animals.

Original languageEnglish
Pages (from-to)341-349
Number of pages9
JournalThrombosis Research
Volume15
Issue number3-4
DOIs
Publication statusPublished - 1979

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

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