Plasmodium lipid rafts contain proteins implicated in vesicular trafficking and signalling as well as members of the PIR superfamily, potentially implicated in host immune system interactions

Francesco Di Girolamo, Carla Raggi, Cecilia Birago, Elisabetta Pizzi, Marco Lalle, Leonardo Picci, Tomasino Pace, Angela Bachi, Joke De Jong, Chris J. Janse, Andrew P. Waters, Massimo Sargiacomo, Marta Ponzi

Research output: Contribution to journalArticle

Abstract

Plasmodium parasites, the causal agents of malaria, dramatically modify the infected erythrocyte by exporting parasite proteins into one or multiple erythrocyte compartments, the cytoplasm and the plasma membrane or beyond. Despite advances in defining signals and specific cellular compartments implicated in protein trafficking in Plasmodium-infected erythrocytes, the contribution of lipid-mediated sorting to this cellular process has been poorly investigated. In this study, we examined the proteome of cholesterol-rich membrane microdomains or lipid rafts, purified from erythrocytes infected by the rodent parasite Plasmodium berghei. Besides structural proteins associated with invasive forms, we detected chaperones, proteins implicated in vesicular trafficking, membrane fusion events and signalling. Interestingly, the raft proteome of mixed P. berghei blood stages included proteins encoded by members of a large family (bir) of putative variant antigens potentially implicated in host immune system interactions and targeted to the surface of the host erythrocytes. The generation of transgenic parasites expressing BIR/GFP fusions confirmed the dynamic association of members of this protein family with membrane microdomains. Our results indicated that lipid rafts in Plasmodium-infected erythrocytes might constitute a route to sort and fold parasite proteins directed to various host cell compartments including the cell surface.

Original languageEnglish
Pages (from-to)2500-2513
Number of pages14
JournalProteomics
Volume8
Issue number12
DOIs
Publication statusPublished - Jun 2008

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Keywords

  • Lipid rafts
  • Plasmodium
  • Vesicular trafficking

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Di Girolamo, F., Raggi, C., Birago, C., Pizzi, E., Lalle, M., Picci, L., Pace, T., Bachi, A., De Jong, J., Janse, C. J., Waters, A. P., Sargiacomo, M., & Ponzi, M. (2008). Plasmodium lipid rafts contain proteins implicated in vesicular trafficking and signalling as well as members of the PIR superfamily, potentially implicated in host immune system interactions. Proteomics, 8(12), 2500-2513. https://doi.org/10.1002/pmic.200700763