Plastic occlusion stress test as a model to investigate the effects of skin delipidization on the stratum corneum water holding capacity in vivo

E. Berardesca, R. Herbst, H. Maibach

Research output: Contribution to journalArticle

Abstract

The purpose of the study was to develop an in vivo model to study the effects of lipid removal on skin barrier. 16 subjects (age 41 ± 8) were delipidized in vivo on the volar forearm using respectively ether/acetone (EA; 1:1) and chloroform/methanol (CM; 2:1). A third site served as control. Water holding capacity (WHC) was measured according to the plastic occlusion stress test (POST) procedure: the water desorption curve after removal of the occlusion was recorded in terms of skin surface water loss (SSWL) using and evaporimeter for 30 min. In the central part of the evaporation curve (bound water) the CM-treated site is significantly different from control and EA-treated sites (p <0.01). The SSWL decay constants reflecting the desorption rate of water from SC are higher in the CM-treated site (p <0.01). The data are consistent with the effect of CM delipidization (polar lipids) on bound water. No differences are recorded in the evaporation of free water. We conclude that polar lipids have a key role in modulating barrier function and WHC of the stratum corneum. The POST can represent a useful in vivo model to study the effects of lipid extraction on skin function.

Original languageEnglish
Pages (from-to)91-94
Number of pages4
JournalDermatology
Volume187
Issue number2
Publication statusPublished - 1993

Fingerprint

Exercise Test
Cornea
Plastics
Skin
Water
Lipids
Chloroform
Acetone
Forearm
Ether
Methanol

Keywords

  • Barrier function
  • Skin lipids
  • Water holding capacity

ASJC Scopus subject areas

  • Dermatology

Cite this

Plastic occlusion stress test as a model to investigate the effects of skin delipidization on the stratum corneum water holding capacity in vivo. / Berardesca, E.; Herbst, R.; Maibach, H.

In: Dermatology, Vol. 187, No. 2, 1993, p. 91-94.

Research output: Contribution to journalArticle

@article{cd7fbbe87027400397aa5a0d256a7212,
title = "Plastic occlusion stress test as a model to investigate the effects of skin delipidization on the stratum corneum water holding capacity in vivo",
abstract = "The purpose of the study was to develop an in vivo model to study the effects of lipid removal on skin barrier. 16 subjects (age 41 ± 8) were delipidized in vivo on the volar forearm using respectively ether/acetone (EA; 1:1) and chloroform/methanol (CM; 2:1). A third site served as control. Water holding capacity (WHC) was measured according to the plastic occlusion stress test (POST) procedure: the water desorption curve after removal of the occlusion was recorded in terms of skin surface water loss (SSWL) using and evaporimeter for 30 min. In the central part of the evaporation curve (bound water) the CM-treated site is significantly different from control and EA-treated sites (p <0.01). The SSWL decay constants reflecting the desorption rate of water from SC are higher in the CM-treated site (p <0.01). The data are consistent with the effect of CM delipidization (polar lipids) on bound water. No differences are recorded in the evaporation of free water. We conclude that polar lipids have a key role in modulating barrier function and WHC of the stratum corneum. The POST can represent a useful in vivo model to study the effects of lipid extraction on skin function.",
keywords = "Barrier function, Skin lipids, Water holding capacity",
author = "E. Berardesca and R. Herbst and H. Maibach",
year = "1993",
language = "English",
volume = "187",
pages = "91--94",
journal = "Dermatology",
issn = "1018-8665",
publisher = "S. Karger AG",
number = "2",

}

TY - JOUR

T1 - Plastic occlusion stress test as a model to investigate the effects of skin delipidization on the stratum corneum water holding capacity in vivo

AU - Berardesca, E.

AU - Herbst, R.

AU - Maibach, H.

PY - 1993

Y1 - 1993

N2 - The purpose of the study was to develop an in vivo model to study the effects of lipid removal on skin barrier. 16 subjects (age 41 ± 8) were delipidized in vivo on the volar forearm using respectively ether/acetone (EA; 1:1) and chloroform/methanol (CM; 2:1). A third site served as control. Water holding capacity (WHC) was measured according to the plastic occlusion stress test (POST) procedure: the water desorption curve after removal of the occlusion was recorded in terms of skin surface water loss (SSWL) using and evaporimeter for 30 min. In the central part of the evaporation curve (bound water) the CM-treated site is significantly different from control and EA-treated sites (p <0.01). The SSWL decay constants reflecting the desorption rate of water from SC are higher in the CM-treated site (p <0.01). The data are consistent with the effect of CM delipidization (polar lipids) on bound water. No differences are recorded in the evaporation of free water. We conclude that polar lipids have a key role in modulating barrier function and WHC of the stratum corneum. The POST can represent a useful in vivo model to study the effects of lipid extraction on skin function.

AB - The purpose of the study was to develop an in vivo model to study the effects of lipid removal on skin barrier. 16 subjects (age 41 ± 8) were delipidized in vivo on the volar forearm using respectively ether/acetone (EA; 1:1) and chloroform/methanol (CM; 2:1). A third site served as control. Water holding capacity (WHC) was measured according to the plastic occlusion stress test (POST) procedure: the water desorption curve after removal of the occlusion was recorded in terms of skin surface water loss (SSWL) using and evaporimeter for 30 min. In the central part of the evaporation curve (bound water) the CM-treated site is significantly different from control and EA-treated sites (p <0.01). The SSWL decay constants reflecting the desorption rate of water from SC are higher in the CM-treated site (p <0.01). The data are consistent with the effect of CM delipidization (polar lipids) on bound water. No differences are recorded in the evaporation of free water. We conclude that polar lipids have a key role in modulating barrier function and WHC of the stratum corneum. The POST can represent a useful in vivo model to study the effects of lipid extraction on skin function.

KW - Barrier function

KW - Skin lipids

KW - Water holding capacity

UR - http://www.scopus.com/inward/record.url?scp=0027227970&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027227970&partnerID=8YFLogxK

M3 - Article

C2 - 8358112

AN - SCOPUS:0027227970

VL - 187

SP - 91

EP - 94

JO - Dermatology

JF - Dermatology

SN - 1018-8665

IS - 2

ER -