TY - JOUR
T1 - Platelet-activating factor-mediated contraction of rabbit lung strips
T2 - Pharmacologic modulation
AU - Camussi, Giovanni
AU - Montrucchio, Giuseppe
AU - Antro, Camillo
AU - Bussolino, Federico
AU - Tetta, Ciro
AU - Emanuelli, Giorgio
PY - 1983
Y1 - 1983
N2 - Synthetic platelet-activating factor (PAF) (1-0-octadecyl-2-acetyl-sn-glyceryl-3-phosphorylcholine, AGEPC) has been shown to induce a slowly developing contraction of rabbit lung parenchymal strips in an isolated organ bath. The spasmogenic effect of AGEPC appeared to be mediated by specific receptors distinct from H1, H2, cholinergic and C5a anaphylatoxin receptors. Prior exposure to AGEPC induced specific desensitization of lung parenchymal strips. Experiments with several pharmacological agents indicated that AGEPC-induced contraction was independent from cyclooxygenase, but was blocked when phosopholipase A2 and lipoxygenase were inhibited and when the Ca++ channels were antagonized. Corticosteroids exhibited an inhibitory effect specific for AGEPC. Intracellular levels of cyclic AMP or cyclic GMP seemed to have a modulatory role in AGEPC-induced contraction of rabbit lung parenchymal strips.
AB - Synthetic platelet-activating factor (PAF) (1-0-octadecyl-2-acetyl-sn-glyceryl-3-phosphorylcholine, AGEPC) has been shown to induce a slowly developing contraction of rabbit lung parenchymal strips in an isolated organ bath. The spasmogenic effect of AGEPC appeared to be mediated by specific receptors distinct from H1, H2, cholinergic and C5a anaphylatoxin receptors. Prior exposure to AGEPC induced specific desensitization of lung parenchymal strips. Experiments with several pharmacological agents indicated that AGEPC-induced contraction was independent from cyclooxygenase, but was blocked when phosopholipase A2 and lipoxygenase were inhibited and when the Ca++ channels were antagonized. Corticosteroids exhibited an inhibitory effect specific for AGEPC. Intracellular levels of cyclic AMP or cyclic GMP seemed to have a modulatory role in AGEPC-induced contraction of rabbit lung parenchymal strips.
KW - Lung contraction
KW - Platelet-activating factor
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U2 - 10.1016/0162-3109(83)90002-4
DO - 10.1016/0162-3109(83)90002-4
M3 - Article
C2 - 6885382
AN - SCOPUS:0020979817
VL - 6
SP - 87
EP - 96
JO - Immunopharmacology
JF - Immunopharmacology
SN - 0162-3109
IS - 2
ER -