Platelet-activating factor-mediated contraction of rabbit lung strips: Pharmacologic modulation

Giovanni Camussi, Giuseppe Montrucchio, Camillo Antro, Federico Bussolino, Ciro Tetta, Giorgio Emanuelli

Research output: Contribution to journalArticlepeer-review

Abstract

Synthetic platelet-activating factor (PAF) (1-0-octadecyl-2-acetyl-sn-glyceryl-3-phosphorylcholine, AGEPC) has been shown to induce a slowly developing contraction of rabbit lung parenchymal strips in an isolated organ bath. The spasmogenic effect of AGEPC appeared to be mediated by specific receptors distinct from H1, H2, cholinergic and C5a anaphylatoxin receptors. Prior exposure to AGEPC induced specific desensitization of lung parenchymal strips. Experiments with several pharmacological agents indicated that AGEPC-induced contraction was independent from cyclooxygenase, but was blocked when phosopholipase A2 and lipoxygenase were inhibited and when the Ca++ channels were antagonized. Corticosteroids exhibited an inhibitory effect specific for AGEPC. Intracellular levels of cyclic AMP or cyclic GMP seemed to have a modulatory role in AGEPC-induced contraction of rabbit lung parenchymal strips.

Original languageEnglish
Pages (from-to)87-96
Number of pages10
JournalImmunopharmacology
Volume6
Issue number2
DOIs
Publication statusPublished - 1983

Keywords

  • Lung contraction
  • Platelet-activating factor

ASJC Scopus subject areas

  • Pharmacology

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