Platelet activating factor (PAF) as a mediator of injury in nephrotoxic nephritis

T. Bertani, M. Livio, D. Macconi, M. Morigi, G. Bisogno, C. Patrono, G. Remuzzi

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Release of acetyl glyceryl ether phosphorylcholine, platelet-activating factor (PAF), has been demonstrated to be associated with glomerular inflammatory damage in acute serum sickness. Moreover, PAF can increase glomerular permeability to proteins and induce mesangial contraction. Thus PAF might be a good candidate as a mediator of glomerular damage. However the in vivo evidence that PAF might cause glomerular injury is lacking. To evaluate if PAF has a major role in promoting glomerular inflammatory reaction and fibrin deposition, we studied the effect of a molecule, L-652,731, which blocks the PAF receptor, on the evolution of an experimental model of anti-glomerular basement membrane (anti-GBM) glomerulonephritis (GN). GN was initiated by sheep-anti-rabbit nephrotoxic serum. A proliferative GN regularly occurred in which heavy proteinuria, intra and extracapillary proliferation of resident and inflammatory cells and fibrin deposition in Bowman's capsule were the prominent findings. Our results showed that the PAF receptor antagonist reduces the glomerular damage in anti-GBM GN, supporting the hypothesis that PAF is indeed a mediator of glomerular inflammatory reaction. PAF receptor blocking prevented renal function deterioration in the early phase of the disease, probably preserving glomerular hemodynamics. In the delayed phase of the disease the PAF receptor antagonist reduced proteinuria and prevented renal function deterioration and fibrin deposition. These effects appear to be mediated by an inhibitory action of PAF receptor blocking on macrophage accumulation and activation.

Original languageEnglish
Pages (from-to)1248-1256
Number of pages9
JournalKidney International
Volume31
Issue number6
Publication statusPublished - 1987

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Nephritis
Platelet Activating Factor
Glomerulonephritis
Wounds and Injuries
Fibrin
Glomerular Basement Membrane
Proteinuria
2,5-bis(3,4,5-trimethoxyphenyl)tetrahydrofuran
Bowman Capsule
Serum Sickness
Kidney
Macrophage Activation
Permeability
Sheep
Theoretical Models
Hemodynamics
platelet activating factor receptor
Rabbits
Serum
Proteins

ASJC Scopus subject areas

  • Nephrology

Cite this

Bertani, T., Livio, M., Macconi, D., Morigi, M., Bisogno, G., Patrono, C., & Remuzzi, G. (1987). Platelet activating factor (PAF) as a mediator of injury in nephrotoxic nephritis. Kidney International, 31(6), 1248-1256.

Platelet activating factor (PAF) as a mediator of injury in nephrotoxic nephritis. / Bertani, T.; Livio, M.; Macconi, D.; Morigi, M.; Bisogno, G.; Patrono, C.; Remuzzi, G.

In: Kidney International, Vol. 31, No. 6, 1987, p. 1248-1256.

Research output: Contribution to journalArticle

Bertani, T, Livio, M, Macconi, D, Morigi, M, Bisogno, G, Patrono, C & Remuzzi, G 1987, 'Platelet activating factor (PAF) as a mediator of injury in nephrotoxic nephritis', Kidney International, vol. 31, no. 6, pp. 1248-1256.
Bertani T, Livio M, Macconi D, Morigi M, Bisogno G, Patrono C et al. Platelet activating factor (PAF) as a mediator of injury in nephrotoxic nephritis. Kidney International. 1987;31(6):1248-1256.
Bertani, T. ; Livio, M. ; Macconi, D. ; Morigi, M. ; Bisogno, G. ; Patrono, C. ; Remuzzi, G. / Platelet activating factor (PAF) as a mediator of injury in nephrotoxic nephritis. In: Kidney International. 1987 ; Vol. 31, No. 6. pp. 1248-1256.
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