Platelet-activating factor production by human fetal microglia - Effect of lipopolysaccharides and tumor necrosis factor-α

Anita Jaranowska, Federico Bussolino, Valeria Sogos, Marco Arese, Giuliana M. Lauro, Fulvia Gremo

Research output: Contribution to journalArticle

Abstract

Since platelet-activating factor (PAF) exerts neurotoxic effects on brain cells, we explored the possibility of PAF production by human fetal microglial cells in vitro. PAF content in pure cultures was assyed and characterized in basic conditions, and after stimulation with growth factors and cytokines. Results showed that microglia cells synthesized PAF when challenged with tumor necrosis factor-α and lipopolysaccharides, whereas other molecules, such as γ-interferon or basic fibroblast growth factor, were ineffective. The induced PAF production was concentration- and time-dependent. These results are in line with the hypothesis that microglia can start a cascade of events leading to tissue damage, thus playing a central role in the pathogenesis of several central nervous system diseases.

Original languageEnglish
Pages (from-to)95-106
Number of pages12
JournalMolecular and Chemical Neuropathology
Volume24
Issue number2-3
DOIs
Publication statusPublished - Feb 1995

Keywords

  • γ-interferon
  • basic fibroblast growth factor
  • cultures
  • cytokines
  • growth factors
  • human microglia
  • lipopolysaccharides (LPS)
  • phorbol esters
  • Platelet-activating factor (PAF)
  • platelet-derived growth factor
  • tumor necrosis factor-α (TNF-α)

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Molecular Biology

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