Platelet activation by cells isolated from human tumor tissues: Effect of cyclooxygenase blockade

L. Pacchiarini, M. Zucchella, F. Tacconi, P. Dionigi, A. Brocchieri, F. Scafa, G. Grignani

Research output: Contribution to journalArticlepeer-review

Abstract

We have studied in a homologous system the effect on different platelet functions of cells isolated from 26 human tumor tissues (11 breast carcinomas, 11 colon carcinomas, 2 pancreatic carcinomas, 1 gastric carcinoma and 1 esophageal carcinoma). Tumor cells (105/ml) significantly increased platelet adhesion to glass beads; they were also found to possess a potent platelet aggregating activity and aggregation was accompanied by significant release of ATP and platelet derived growth factor (PDGF) and by production of TXB2. Preincubation of platelets with a low concentration (1 μM) of indobufen, a cyclooxygenase inhibitor, significantly reduced tumor cell induced TXB2 production and ATP release, while the other platelet functions were not modified. Higher concentrations of the drug (10 or 100 μM) were also able to inhibit tumor cell-induced platelet aggregation and PDGF release, while platelet adhesion to glass beads was unchanged even at these doses. Finally, preincubation of neoplastic cells with indobufen (400μM) had no effect on their ability to induce platelet aggregation, TXB2 production and ATP release. These data demonstrate that cyclooxygenase blockade in platelets has different effects on several platelet functions activated by the tumor cells that were investigated.

Original languageEnglish
Pages (from-to)207-211
Number of pages5
JournalPlatelets
Volume4
Issue number4
DOIs
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Hematology
  • Cell Biology

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