Platelet aggregation and haemolysis induced in rats by intravenous infusion of ADP. Effect of potentially antithrombotic drugs

This paper describes a simple experimental model to quantitate the in vivo aggregation of rat platelets induced by ADP and the in vivo effectiveness of compounds with potential anti aggregating activity; only drugs known to inhibit in vitro the first phases of platelet aggregation (PGE₁, pyrimido pyrimidine compounds) were inhibitory in this model; in contrast, acetylsalicylic acid, known to inhibit in vitro the last phases of platelet aggregation, was ineffective; no interaction between pyrimido pyrimidine compounds and acetylsalicylic acid was observed. Besides platelet aggregation, ADP also induced a marked hemolysis; the latter was not inhibited by any of the drugs tested.