Platelet aggregation studies: Autologous platelet-poor plasma inhibits platelet aggregation when added to platelet-rich plasma to normalize platelet count

Marco Cattaneo, Anna Lecchi, Maddalena Loredana Zighetti, Federico Lussana

Research output: Contribution to journalArticlepeer-review

Abstract

Adjusting platelet count (PC) in platelet-rich plasma (PRP) using platelet-poor plasma (PPP) is recommended for platelet aggregation (PA) studies, but it could also affect PA independently of the decrease in PC. Analysis of aggregation tracings from healthy controls showed that PC correlated with PA in 47 diluted-PRPs, but not in 104 undiluted-PRPs. Dilution of 9 PRPs with PPP progressively decreased PA, while dilution of washed platelets with buffer hardly affected PA. Apyrase partially prevented the inhibitory effect of PPP. Therefore, the practice of diluting PRP with PPP to adjust platelet count should be avoided because it artefactually inhibits PA.

Original languageEnglish
Pages (from-to)694-697
Number of pages4
JournalHaematologica
Volume92
Issue number5
DOIs
Publication statusPublished - May 2007

Keywords

  • Platelet aggregation
  • Platelet count
  • Platelet-rich plasma

ASJC Scopus subject areas

  • Hematology

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