Abstract
The haemocompatibility of polyethylene terephthalate (Dacron®) coated with pyrolytic carbon was examined in vitro, evaluating its capability of inducing adhesion and platelet activation, and of modifying the intrinsic coagulation pathway. Platelet adhesion was evaluated by counting platelets before and after in vitro contact of human plasma with the material under examination. Platelet activation was evaluated by determining platelet factor 4 (PF4) and thromboxane B2. Intrinsic coagulation pathway alterations were studied by determining activated partial thromboplastin time (APTT) and the activity of single factors. The results obtained show that pyrolytic carbon-coated Dacron induces platelet adhesion, reduction in platelet volume and lower increase in thromboxane production than that obtained after contact with uncoated Dacron. Pyrolytic carbon-coated Dacron does not induce PF4 release, contrary to uncoated Dacron induces a significant release. Moreover, pyrolytic carbon-coated Dacron, induces a significant extension of APTT by reducing the activity of intrinsic pathway factors, particularly factor XI.
| Original language | English |
|---|---|
| Pages (from-to) | 973-976 |
| Number of pages | 4 |
| Journal | Biomaterials |
| Volume | 16 |
| Issue number | 13 |
| DOIs | |
| Publication status | Published - 1995 |
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Keywords
- intrinsic coagulation pathway
- platelets
- Pyrolytic carbon
ASJC Scopus subject areas
- Biophysics
- Bioengineering
- Mechanics of Materials
- Biomaterials
- Ceramics and Composites
- Biotechnology
- Biomedical Engineering
Cite this
Platelet and coagulation factor variations induced in vitro by polyethylene terephthalate (Dacron®) coated with pyrolytic carbon. / Cenni, Elisabetta; Arciola, Carla Renata; Ciapetti, Gabriela; Granchi, Donatella; Savarino, Lucia; Stea, Susanna; Cavedagna, Daniela; Curti, Tiziano; Falsone, Giuseppina; Pizzoferrato, Arturo.
In: Biomaterials, Vol. 16, No. 13, 1995, p. 973-976.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Platelet and coagulation factor variations induced in vitro by polyethylene terephthalate (Dacron®) coated with pyrolytic carbon
AU - Cenni, Elisabetta
AU - Arciola, Carla Renata
AU - Ciapetti, Gabriela
AU - Granchi, Donatella
AU - Savarino, Lucia
AU - Stea, Susanna
AU - Cavedagna, Daniela
AU - Curti, Tiziano
AU - Falsone, Giuseppina
AU - Pizzoferrato, Arturo
PY - 1995
Y1 - 1995
N2 - The haemocompatibility of polyethylene terephthalate (Dacron®) coated with pyrolytic carbon was examined in vitro, evaluating its capability of inducing adhesion and platelet activation, and of modifying the intrinsic coagulation pathway. Platelet adhesion was evaluated by counting platelets before and after in vitro contact of human plasma with the material under examination. Platelet activation was evaluated by determining platelet factor 4 (PF4) and thromboxane B2. Intrinsic coagulation pathway alterations were studied by determining activated partial thromboplastin time (APTT) and the activity of single factors. The results obtained show that pyrolytic carbon-coated Dacron induces platelet adhesion, reduction in platelet volume and lower increase in thromboxane production than that obtained after contact with uncoated Dacron. Pyrolytic carbon-coated Dacron does not induce PF4 release, contrary to uncoated Dacron induces a significant release. Moreover, pyrolytic carbon-coated Dacron, induces a significant extension of APTT by reducing the activity of intrinsic pathway factors, particularly factor XI.
AB - The haemocompatibility of polyethylene terephthalate (Dacron®) coated with pyrolytic carbon was examined in vitro, evaluating its capability of inducing adhesion and platelet activation, and of modifying the intrinsic coagulation pathway. Platelet adhesion was evaluated by counting platelets before and after in vitro contact of human plasma with the material under examination. Platelet activation was evaluated by determining platelet factor 4 (PF4) and thromboxane B2. Intrinsic coagulation pathway alterations were studied by determining activated partial thromboplastin time (APTT) and the activity of single factors. The results obtained show that pyrolytic carbon-coated Dacron induces platelet adhesion, reduction in platelet volume and lower increase in thromboxane production than that obtained after contact with uncoated Dacron. Pyrolytic carbon-coated Dacron does not induce PF4 release, contrary to uncoated Dacron induces a significant release. Moreover, pyrolytic carbon-coated Dacron, induces a significant extension of APTT by reducing the activity of intrinsic pathway factors, particularly factor XI.
KW - intrinsic coagulation pathway
KW - platelets
KW - Pyrolytic carbon
UR - http://www.scopus.com/inward/record.url?scp=0029361222&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029361222&partnerID=8YFLogxK
U2 - 10.1016/0142-9612(95)94903-X
DO - 10.1016/0142-9612(95)94903-X
M3 - Article
C2 - 8580259
AN - SCOPUS:0029361222
VL - 16
SP - 973
EP - 976
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 13
ER -