Platelet and coagulation factor variations induced in vitro by polyethylene terephthalate (Dacron®) coated with pyrolytic carbon

Elisabetta Cenni, Carla Renata Arciola, Gabriela Ciapetti, Donatella Granchi, Lucia Savarino, Susanna Stea, Daniela Cavedagna, Tiziano Curti, Giuseppina Falsone, Arturo Pizzoferrato

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The haemocompatibility of polyethylene terephthalate (Dacron®) coated with pyrolytic carbon was examined in vitro, evaluating its capability of inducing adhesion and platelet activation, and of modifying the intrinsic coagulation pathway. Platelet adhesion was evaluated by counting platelets before and after in vitro contact of human plasma with the material under examination. Platelet activation was evaluated by determining platelet factor 4 (PF4) and thromboxane B2. Intrinsic coagulation pathway alterations were studied by determining activated partial thromboplastin time (APTT) and the activity of single factors. The results obtained show that pyrolytic carbon-coated Dacron induces platelet adhesion, reduction in platelet volume and lower increase in thromboxane production than that obtained after contact with uncoated Dacron. Pyrolytic carbon-coated Dacron does not induce PF4 release, contrary to uncoated Dacron induces a significant release. Moreover, pyrolytic carbon-coated Dacron, induces a significant extension of APTT by reducing the activity of intrinsic pathway factors, particularly factor XI.

Original languageEnglish
Pages (from-to)973-976
Number of pages4
JournalBiomaterials
Volume16
Issue number13
DOIs
Publication statusPublished - 1995

Fingerprint

Polyethylene Terephthalates
Blood Coagulation Factors
Platelets
Coagulation
Polyethylene terephthalates
Blood Platelets
Carbon
Platelet Factor 4
Adhesion
Partial Thromboplastin Time
Platelet Activation
Thromboplastin
Chemical activation
Factor XI
Plasma (human)
Thromboxane B2
Intrinsic Factor
Thromboxanes
pyrolytic carbon
In Vitro Techniques

Keywords

  • intrinsic coagulation pathway
  • platelets
  • Pyrolytic carbon

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Mechanics of Materials
  • Biomaterials
  • Ceramics and Composites
  • Biotechnology
  • Biomedical Engineering

Cite this

Platelet and coagulation factor variations induced in vitro by polyethylene terephthalate (Dacron®) coated with pyrolytic carbon. / Cenni, Elisabetta; Arciola, Carla Renata; Ciapetti, Gabriela; Granchi, Donatella; Savarino, Lucia; Stea, Susanna; Cavedagna, Daniela; Curti, Tiziano; Falsone, Giuseppina; Pizzoferrato, Arturo.

In: Biomaterials, Vol. 16, No. 13, 1995, p. 973-976.

Research output: Contribution to journalArticle

Cenni, Elisabetta ; Arciola, Carla Renata ; Ciapetti, Gabriela ; Granchi, Donatella ; Savarino, Lucia ; Stea, Susanna ; Cavedagna, Daniela ; Curti, Tiziano ; Falsone, Giuseppina ; Pizzoferrato, Arturo. / Platelet and coagulation factor variations induced in vitro by polyethylene terephthalate (Dacron®) coated with pyrolytic carbon. In: Biomaterials. 1995 ; Vol. 16, No. 13. pp. 973-976.
@article{cc068b2d05ab4d32b68b9dc69857fa0b,
title = "Platelet and coagulation factor variations induced in vitro by polyethylene terephthalate (Dacron{\circledR}) coated with pyrolytic carbon",
abstract = "The haemocompatibility of polyethylene terephthalate (Dacron{\circledR}) coated with pyrolytic carbon was examined in vitro, evaluating its capability of inducing adhesion and platelet activation, and of modifying the intrinsic coagulation pathway. Platelet adhesion was evaluated by counting platelets before and after in vitro contact of human plasma with the material under examination. Platelet activation was evaluated by determining platelet factor 4 (PF4) and thromboxane B2. Intrinsic coagulation pathway alterations were studied by determining activated partial thromboplastin time (APTT) and the activity of single factors. The results obtained show that pyrolytic carbon-coated Dacron induces platelet adhesion, reduction in platelet volume and lower increase in thromboxane production than that obtained after contact with uncoated Dacron. Pyrolytic carbon-coated Dacron does not induce PF4 release, contrary to uncoated Dacron induces a significant release. Moreover, pyrolytic carbon-coated Dacron, induces a significant extension of APTT by reducing the activity of intrinsic pathway factors, particularly factor XI.",
keywords = "intrinsic coagulation pathway, platelets, Pyrolytic carbon",
author = "Elisabetta Cenni and Arciola, {Carla Renata} and Gabriela Ciapetti and Donatella Granchi and Lucia Savarino and Susanna Stea and Daniela Cavedagna and Tiziano Curti and Giuseppina Falsone and Arturo Pizzoferrato",
year = "1995",
doi = "10.1016/0142-9612(95)94903-X",
language = "English",
volume = "16",
pages = "973--976",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "13",

}

TY - JOUR

T1 - Platelet and coagulation factor variations induced in vitro by polyethylene terephthalate (Dacron®) coated with pyrolytic carbon

AU - Cenni, Elisabetta

AU - Arciola, Carla Renata

AU - Ciapetti, Gabriela

AU - Granchi, Donatella

AU - Savarino, Lucia

AU - Stea, Susanna

AU - Cavedagna, Daniela

AU - Curti, Tiziano

AU - Falsone, Giuseppina

AU - Pizzoferrato, Arturo

PY - 1995

Y1 - 1995

N2 - The haemocompatibility of polyethylene terephthalate (Dacron®) coated with pyrolytic carbon was examined in vitro, evaluating its capability of inducing adhesion and platelet activation, and of modifying the intrinsic coagulation pathway. Platelet adhesion was evaluated by counting platelets before and after in vitro contact of human plasma with the material under examination. Platelet activation was evaluated by determining platelet factor 4 (PF4) and thromboxane B2. Intrinsic coagulation pathway alterations were studied by determining activated partial thromboplastin time (APTT) and the activity of single factors. The results obtained show that pyrolytic carbon-coated Dacron induces platelet adhesion, reduction in platelet volume and lower increase in thromboxane production than that obtained after contact with uncoated Dacron. Pyrolytic carbon-coated Dacron does not induce PF4 release, contrary to uncoated Dacron induces a significant release. Moreover, pyrolytic carbon-coated Dacron, induces a significant extension of APTT by reducing the activity of intrinsic pathway factors, particularly factor XI.

AB - The haemocompatibility of polyethylene terephthalate (Dacron®) coated with pyrolytic carbon was examined in vitro, evaluating its capability of inducing adhesion and platelet activation, and of modifying the intrinsic coagulation pathway. Platelet adhesion was evaluated by counting platelets before and after in vitro contact of human plasma with the material under examination. Platelet activation was evaluated by determining platelet factor 4 (PF4) and thromboxane B2. Intrinsic coagulation pathway alterations were studied by determining activated partial thromboplastin time (APTT) and the activity of single factors. The results obtained show that pyrolytic carbon-coated Dacron induces platelet adhesion, reduction in platelet volume and lower increase in thromboxane production than that obtained after contact with uncoated Dacron. Pyrolytic carbon-coated Dacron does not induce PF4 release, contrary to uncoated Dacron induces a significant release. Moreover, pyrolytic carbon-coated Dacron, induces a significant extension of APTT by reducing the activity of intrinsic pathway factors, particularly factor XI.

KW - intrinsic coagulation pathway

KW - platelets

KW - Pyrolytic carbon

UR - http://www.scopus.com/inward/record.url?scp=0029361222&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029361222&partnerID=8YFLogxK

U2 - 10.1016/0142-9612(95)94903-X

DO - 10.1016/0142-9612(95)94903-X

M3 - Article

C2 - 8580259

AN - SCOPUS:0029361222

VL - 16

SP - 973

EP - 976

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 13

ER -