Platelet and leukocyte ROS production and lipoperoxidation are associated with high platelet reactivity in Non-ST elevation myocardial infarction (NSTEMI) patients on dual antiplatelet treatment

Matteo Becatti, Claudia Fiorillo, Anna Maria Gori, Rossella Marcucci, Rita Paniccia, Betti Giusti, Francesco Violi, Pasquale Pignatelli, Gian Franco Gensini, Rosanna Abbate

Research output: Contribution to journalArticle

Abstract

Introduction: High platelet reactivity (HPR) on dual antiplatelet therapy is a risk factor for adverse vascular events in acute coronary syndrome (ACS) patients. Several studies have shown that reactive oxygen species (ROS) may be involved in modulating platelet function. Methods: In Non-ST elevation myocardial infarction (NSTEMI) patients ( n=132) undergoing percutaneous coronary intervention (PCI) on dual antiplatelet therapy blood samples were collected within 24h from 600mg clopidogrel loading dose. Platelet reactivity was assessed by light transmission aggregometry using 10μM ADP, 1mM arachidonic acid (AA) and 2μg/ml collagen. ROS production and lipoperoxidation of circulating cells were determined. by FACSCanto flow cytometry. In these patients, we investigated: 1) the relationship between the amount of cellular ROS production/lipoperoxidation and platelet reactivity; 2) the association of cellular ROS production with the presence of high platelet reactivity to ADP and arachidonic acid (AA). Results: Significantly higher levels of platelet and leukocyte-derived ROS were detected in 49 dual HPR (with platelet aggregation by AA≥20% and by ADP≥70%) compared to non-HPR patients ( n=49) [Platelet-derived ROS:+142%; Leukocyte-derived ROS:+14%, p

Original languageEnglish
Pages (from-to)392-400
Number of pages9
JournalAtherosclerosis
Volume231
Issue number2
DOIs
Publication statusPublished - Dec 2013

Keywords

  • High platelet reactivity
  • Leukocytes
  • Lipoperoxidation
  • ROS production

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Fingerprint Dive into the research topics of 'Platelet and leukocyte ROS production and lipoperoxidation are associated with high platelet reactivity in Non-ST elevation myocardial infarction (NSTEMI) patients on dual antiplatelet treatment'. Together they form a unique fingerprint.

  • Cite this