Platelet arachidonic acid metabolism in patients with cardiovascular disorders

D. Ghigo, S. Treves, F. Bussolino, L. Libero, F. Orzan, M. Bazzan, A. Pannocchia, G. Tamponi, A. Bosia

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Abstract

There is increasing evidence that prostaglandins (PG) and thromboxane (Tx) play a major role in the pathogenesis of coronary artery disease. The regulation of arachidonic acid (AA) metabolism through cyclooxygenase (COx) pathway and the AA-dependent Ca2+ influx were investigated in platelets from 10 patients with unstable angina and 10 controls. The activation of the hexose monophosphate shunt (HMS), a sensitive index of the flux through the PGG2 to PGH2 step of the COx pathway, in response to AA was significantly enhanced in platelets from patients. AA-induced malonyldialdehyde (MDA) production as well as AA-evoked Ca2+ flux and glutathione-dependent peroxidase activity resulted significantly increased. Moreover, platelet sensitivity to prostacyclin (PGI2), measured as inhibition of Ca2+ flux, was highly decreased. Thus far, evidence is presented for intrinsic platelet hyperactivity (at the PG-peroxidase reaction of the COx pathway) in patients with unstable angina: the resulting increase in PGH2 and TxA2 synthesis, alone or in combination with decreased PGI2 sensitivity, may account for a facilitated thrombus formation.

Original languageEnglish
JournalBiomedica Biochimica Acta
Volume47
Issue number10-11
Publication statusPublished - 1988

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ASJC Scopus subject areas

  • Biochemistry

Cite this

Ghigo, D., Treves, S., Bussolino, F., Libero, L., Orzan, F., Bazzan, M., Pannocchia, A., Tamponi, G., & Bosia, A. (1988). Platelet arachidonic acid metabolism in patients with cardiovascular disorders. Biomedica Biochimica Acta, 47(10-11).