Platelet calcium homeostasis is abnormal in patients with severe arteriosclerosis

Aurelio M. Vicari, Maria Luisa Monzani, Fabio Pellegatta, Paola Ronchi, Laura Galli, Franco Folli

Research output: Contribution to journalArticle

Abstract

To evaluate platelet calcium homeostasis in a typical thrombosis-prone clinical condition, 14 patients with severe arteriosclerosis and 11 healthy control subjects were studied. Platelet intracellular free calcium concentration ([Ca2+]i) was evaluated by means of the fluorescent probe fura 2 under resting conditions and after challenge with 0.05, 0.1, and 0.5 U/mL thrombin (final concentrations). Three different concentrations of extracellular ionized calcium ([Ca2+]e) were used: 1 mmol/L, 1 μmol/L, and 2+]i was significantly higher (P2+]i was lower in patients than in control subjects in each of the three [Ca2+]i conditions (P=.05 at 1 mmol/L, P=.02 at 1 μmol/L, and P=.04 at 2+], was lower in patients than in control subjects under two [Ca2+]c conditions, 1 μmol/L and 2+]c. These results suggest that calcium homeostasis is abnormal in platelets from patients with severe arteriosclerosis and probably reflects a chronic activation.

Original languageEnglish
Pages (from-to)1420-1424
Number of pages5
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume14
Issue number9
Publication statusPublished - 1994

Keywords

  • Arteriosclerosis
  • Ionized calcium
  • Platelets

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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  • Cite this

    Vicari, A. M., Monzani, M. L., Pellegatta, F., Ronchi, P., Galli, L., & Folli, F. (1994). Platelet calcium homeostasis is abnormal in patients with severe arteriosclerosis. Arteriosclerosis, Thrombosis, and Vascular Biology, 14(9), 1420-1424.