Platelet calcium homeostasis is abnormal in patients with severe arteriosclerosis

Aurelio M. Vicari, Maria Luisa Monzani, Fabio Pellegatta, Paola Ronchi, Laura Galli, Franco Folli

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

To evaluate platelet calcium homeostasis in a typical thrombosis-prone clinical condition, 14 patients with severe arteriosclerosis and 11 healthy control subjects were studied. Platelet intracellular free calcium concentration ([Ca2+]i) was evaluated by means of the fluorescent probe fura 2 under resting conditions and after challenge with 0.05, 0.1, and 0.5 U/mL thrombin (final concentrations). Three different concentrations of extracellular ionized calcium ([Ca2+]e) were used: 1 mmol/L, 1 μmol/L, and 2+]i was significantly higher (P2+]i was lower in patients than in control subjects in each of the three [Ca2+]i conditions (P=.05 at 1 mmol/L, P=.02 at 1 μmol/L, and P=.04 at 2+], was lower in patients than in control subjects under two [Ca2+]c conditions, 1 μmol/L and 2+]c. These results suggest that calcium homeostasis is abnormal in platelets from patients with severe arteriosclerosis and probably reflects a chronic activation.

Original languageEnglish
Pages (from-to)1420-1424
Number of pages5
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume14
Issue number9
Publication statusPublished - 1994

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Arteriosclerosis
Homeostasis
Blood Platelets
Calcium
Fura-2
Fluorescent Dyes
Thrombin
Healthy Volunteers
Thrombosis

Keywords

  • Arteriosclerosis
  • Ionized calcium
  • Platelets

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Vicari, A. M., Monzani, M. L., Pellegatta, F., Ronchi, P., Galli, L., & Folli, F. (1994). Platelet calcium homeostasis is abnormal in patients with severe arteriosclerosis. Arteriosclerosis, Thrombosis, and Vascular Biology, 14(9), 1420-1424.

Platelet calcium homeostasis is abnormal in patients with severe arteriosclerosis. / Vicari, Aurelio M.; Monzani, Maria Luisa; Pellegatta, Fabio; Ronchi, Paola; Galli, Laura; Folli, Franco.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 14, No. 9, 1994, p. 1420-1424.

Research output: Contribution to journalArticle

Vicari, AM, Monzani, ML, Pellegatta, F, Ronchi, P, Galli, L & Folli, F 1994, 'Platelet calcium homeostasis is abnormal in patients with severe arteriosclerosis', Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 14, no. 9, pp. 1420-1424.
Vicari, Aurelio M. ; Monzani, Maria Luisa ; Pellegatta, Fabio ; Ronchi, Paola ; Galli, Laura ; Folli, Franco. / Platelet calcium homeostasis is abnormal in patients with severe arteriosclerosis. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 1994 ; Vol. 14, No. 9. pp. 1420-1424.
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