To evaluate platelet calcium homeostasis in a typical thrombosis-prone clinical condition, 14 patients with severe arteriosclerosis and 11 healthy control subjects were studied. Platelet intracellular free calcium concentration ([Ca2+]i) was evaluated by means of the fluorescent probe fura 2 under resting conditions and after challenge with 0.05, 0.1, and 0.5 U/mL thrombin (final concentrations). Three different concentrations of extracellular ionized calcium ([Ca2+]e) were used: 1 mmol/L, 1 μmol/L, and 2+]i was significantly higher (P2+]i was lower in patients than in control subjects in each of the three [Ca2+]i conditions (P=.05 at 1 mmol/L, P=.02 at 1 μmol/L, and P=.04 at 2+], was lower in patients than in control subjects under two [Ca2+]c conditions, 1 μmol/L and 2+]c. These results suggest that calcium homeostasis is abnormal in platelets from patients with severe arteriosclerosis and probably reflects a chronic activation.
|Number of pages||5|
|Journal||Arteriosclerosis, Thrombosis, and Vascular Biology|
|Publication status||Published - 1994|
- Ionized calcium
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine