Platelet concentrate prepared from pooled buffy coats (BCPC) were stored in Plasma-Lyte A, a glucose-free synthetic medium, after leukocyte depletion by filtration through Pall PL50, and compared to paired unfiltered BCPC stored in the same medium. Each pair of BCPC units was prepared from a pool of 10 buffy coats split into two identical unites. Platelet and leukocyte counts per unit of BCPC were 2.70 ± 0.19 X 1011 and 3.8 ± 2.8 X 106 (filtered BCPC), 2.59 ± 0.27 X 1011 and 79 ± 56 X 106 (control BCPC), respectively. Filtration procedures did not affect in vitro parameters of platelet quality and function such as osmotic reversal, ATP release and aggregation in response to collagen and ADP during 15-day storage. A similar decrease of platelet membrane glycoprotein Ib and a similar rise of activation markers GMP-140, gp 53 and platelet-bound fibrogen were observed during storage of filtered and control BCPC. Our study indicates that storage of BCPC after filtration is feasible and that a reduction in leukocyte content by filtration to mean cell counts of less than five million per unit has probably no effect on platelet storage lesion.
|Number of pages||5|
|Publication status||Published - 1992|
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