TY - JOUR
T1 - Platelet count may impact on lysosomal acid lipase activity determination in dried blood spot
AU - Vespasiani-Gentilucci, Umberto
AU - D'Amico, Jessica
AU - De Vincentis, Antonio
AU - Tozzi, Giulia
AU - Vorini, Ferruccio
AU - Gallo, Paolo
AU - Carotti, Simone
AU - Valentini, Francesco
AU - Galati, Giovanni
AU - dell'Unto, Chiara
AU - Piemonte, Fiorella
AU - Picardi, Antonio
PY - 2017/8/1
Y1 - 2017/8/1
N2 - Background We aimed to evaluate the influence of white blood cell (WBC) and platelet (PLT) counts on dried blood spot (DBS)-determined lysosomal acid lipase (LAL) activity in a large group of healthy subjects. Methods One-hundred-and-seventy-two healthy subjects aged ≥ 18 were enrolled. Complete clinical biochemistry and LAL activity in DBS were determined. In 35 subjects, WBCs and PLTs were isolated, and LAL activity was measured in both blood cell populations. Univariate and multivariate analyses to DBS-LAL activity were performed. Results Mean age of subjects was 44.8 ± 17.2 years, 43.6% were males, and mean DBS-LAL activity was normal (1.0 ± 0.3 nmol/spot/h). LAL activity in WBCs was significantly higher than in PLTs (458.9 ± 133.6 vs 235.0 ± 88.3 nmol/mg/h, p < 0.001). However, LAL activity in DBS correlated more strongly with that in PLTs (r = 0.65, p < 0.001) than with that in WBCs (r = 0.49, p < 0.01). Consistently, in the multivariate model, DBS-LAL activity was independently associated only with PLT count (β = 0.39, p < 0.001). Conclusions PLT number may impact on the result of the DBS-LAL test, and a consideration of PLT count is recommended before interpreting LAL activity in DBS.
AB - Background We aimed to evaluate the influence of white blood cell (WBC) and platelet (PLT) counts on dried blood spot (DBS)-determined lysosomal acid lipase (LAL) activity in a large group of healthy subjects. Methods One-hundred-and-seventy-two healthy subjects aged ≥ 18 were enrolled. Complete clinical biochemistry and LAL activity in DBS were determined. In 35 subjects, WBCs and PLTs were isolated, and LAL activity was measured in both blood cell populations. Univariate and multivariate analyses to DBS-LAL activity were performed. Results Mean age of subjects was 44.8 ± 17.2 years, 43.6% were males, and mean DBS-LAL activity was normal (1.0 ± 0.3 nmol/spot/h). LAL activity in WBCs was significantly higher than in PLTs (458.9 ± 133.6 vs 235.0 ± 88.3 nmol/mg/h, p < 0.001). However, LAL activity in DBS correlated more strongly with that in PLTs (r = 0.65, p < 0.001) than with that in WBCs (r = 0.49, p < 0.01). Consistently, in the multivariate model, DBS-LAL activity was independently associated only with PLT count (β = 0.39, p < 0.001). Conclusions PLT number may impact on the result of the DBS-LAL test, and a consideration of PLT count is recommended before interpreting LAL activity in DBS.
KW - Dried blood spot
KW - Lysosomal acid lipase
KW - Platelets
KW - White blood cells
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U2 - 10.1016/j.clinbiochem.2017.02.013
DO - 10.1016/j.clinbiochem.2017.02.013
M3 - Article
C2 - 28238812
AN - SCOPUS:85014068033
VL - 50
SP - 726
EP - 728
JO - Clinical Biochemistry
JF - Clinical Biochemistry
SN - 0009-9120
IS - 12
ER -