TY - JOUR
T1 - Platelet derived growth factor B gene expression in the Xenopus laevis developing central nervous system
AU - Giannetti, Kety
AU - Corsinovi, Debora
AU - Rossino, Cristina
AU - Appolloni, Irene
AU - Malatesta, Paolo
AU - Ori, Michela
PY - 2016
Y1 - 2016
N2 - Platelet-derived growth factor B (PDGF-B) belongs to the mitogen and growth factor family and like the other members it has many roles in cell differentiation, proliferation and migration during development, adult life and in pathological conditions. Among them it has been observed that aberrant PDGF signalling is frequently linked to glioma development and progression, and Pdgf-b over-expression in mouse neural progenitors leads to the formation of gliomas. Despite this evidence, the mechanisms underlying PDGF-B driven tumorigenesis and its role during brain development are not fully understood. In order to contribute to clarifying possible new roles of pdgf-b signalling, we present here the embryonic gene expression pattern of pdgf-b, so far unknown in early vertebrate development. By using Xenopus laevis as a model system we performed qRT-PCR and whole mount in situ hybridization. Pdgf-b mRNA is expressed in discrete regions of the developing central nervous system, in the cranial nerve placodes and in the notochord. We also compared the gene expression of pdgf-b with that of its receptor pdgfr-a suggesting so far unsuspected roles for this signalling pathway during the development of specific embryonic structures.
AB - Platelet-derived growth factor B (PDGF-B) belongs to the mitogen and growth factor family and like the other members it has many roles in cell differentiation, proliferation and migration during development, adult life and in pathological conditions. Among them it has been observed that aberrant PDGF signalling is frequently linked to glioma development and progression, and Pdgf-b over-expression in mouse neural progenitors leads to the formation of gliomas. Despite this evidence, the mechanisms underlying PDGF-B driven tumorigenesis and its role during brain development are not fully understood. In order to contribute to clarifying possible new roles of pdgf-b signalling, we present here the embryonic gene expression pattern of pdgf-b, so far unknown in early vertebrate development. By using Xenopus laevis as a model system we performed qRT-PCR and whole mount in situ hybridization. Pdgf-b mRNA is expressed in discrete regions of the developing central nervous system, in the cranial nerve placodes and in the notochord. We also compared the gene expression of pdgf-b with that of its receptor pdgfr-a suggesting so far unsuspected roles for this signalling pathway during the development of specific embryonic structures.
KW - Central nervous system
KW - Neural crest
KW - PDGF receptor α
KW - PDGF-B
KW - Xenopus laevis
UR - http://www.scopus.com/inward/record.url?scp=84977491604&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84977491604&partnerID=8YFLogxK
U2 - 10.1387/ijdb.160045mo
DO - 10.1387/ijdb.160045mo
M3 - Article
AN - SCOPUS:84977491604
VL - 60
SP - 175
EP - 179
JO - International Journal of Developmental Biology
JF - International Journal of Developmental Biology
SN - 0214-6282
IS - 4-6
ER -