Platelet derived growth factor B gene expression in the Xenopus laevis developing central nervous system

Kety Giannetti, Debora Corsinovi, Cristina Rossino, Irene Appolloni, Paolo Malatesta, Michela Ori

Research output: Contribution to journalArticlepeer-review

Abstract

Platelet-derived growth factor B (PDGF-B) belongs to the mitogen and growth factor family and like the other members it has many roles in cell differentiation, proliferation and migration during development, adult life and in pathological conditions. Among them it has been observed that aberrant PDGF signalling is frequently linked to glioma development and progression, and Pdgf-b over-expression in mouse neural progenitors leads to the formation of gliomas. Despite this evidence, the mechanisms underlying PDGF-B driven tumorigenesis and its role during brain development are not fully understood. In order to contribute to clarifying possible new roles of pdgf-b signalling, we present here the embryonic gene expression pattern of pdgf-b, so far unknown in early vertebrate development. By using Xenopus laevis as a model system we performed qRT-PCR and whole mount in situ hybridization. Pdgf-b mRNA is expressed in discrete regions of the developing central nervous system, in the cranial nerve placodes and in the notochord. We also compared the gene expression of pdgf-b with that of its receptor pdgfr-a suggesting so far unsuspected roles for this signalling pathway during the development of specific embryonic structures.

Original languageEnglish
Pages (from-to)175-179
Number of pages5
JournalInternational Journal of Developmental Biology
Volume60
Issue number4-6
DOIs
Publication statusPublished - 2016

Keywords

  • Central nervous system
  • Neural crest
  • PDGF receptor α
  • PDGF-B
  • Xenopus laevis

ASJC Scopus subject areas

  • Developmental Biology
  • Embryology

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