Expression of the platelet-derived growth factor receptor (PDGFR) was detected by immunocytochemistry in normal and malignant mesothelial cells from 14 benign effusions and 22 mesotheliomas. Two well-characterized antireceptor monoclonal antibodies to the PDGFR α-subunit (PR292) and β- subunit (PR7212) were used. PDGFR α-subunit outlined cell membranes intensely in nonneoplastic mesothelial cells, whereas it was focal in mesothelioma and limited to a few cases only. PDGFR β-subunit was weakly expressed in the cytoplasm of normal mesothelium; in contrast, malignant mesothelial cells showed strong cytoplasmic staining. Our results show that normal mesothelium may be responsive to PDGF by the predominant expression of PDGFR-α and less by PDGFR-β and indicate the presence of growth-stimulation loops in mesothelioma through PDGF/PDGFR-β interaction. Also, scattered staining for the PDGFR α-subunit suggests that a PDGF-A chain/PDGFR-α interaction may also be active in mesothelioma cells growing in suspension.
|Number of pages||10|
|Publication status||Published - 1995|
- platelet-derived growth factor
ASJC Scopus subject areas
- Cell Biology