Platelet-derived growth factor receptor immunoreactivity in mesothelioma and nonneoplastic mesothelial cells in serous effusions

V. Ascoli, C. C. Scalzo, F. Facciolo, F. Nardi

Research output: Contribution to journalArticle

Abstract

Expression of the platelet-derived growth factor receptor (PDGFR) was detected by immunocytochemistry in normal and malignant mesothelial cells from 14 benign effusions and 22 mesotheliomas. Two well-characterized antireceptor monoclonal antibodies to the PDGFR α-subunit (PR292) and β- subunit (PR7212) were used. PDGFR α-subunit outlined cell membranes intensely in nonneoplastic mesothelial cells, whereas it was focal in mesothelioma and limited to a few cases only. PDGFR β-subunit was weakly expressed in the cytoplasm of normal mesothelium; in contrast, malignant mesothelial cells showed strong cytoplasmic staining. Our results show that normal mesothelium may be responsive to PDGF by the predominant expression of PDGFR-α and less by PDGFR-β and indicate the presence of growth-stimulation loops in mesothelioma through PDGF/PDGFR-β interaction. Also, scattered staining for the PDGFR α-subunit suggests that a PDGF-A chain/PDGFR-α interaction may also be active in mesothelioma cells growing in suspension.

Original languageEnglish
Pages (from-to)613-622
Number of pages10
JournalActa Cytologica
Volume39
Issue number4
Publication statusPublished - 1995

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Keywords

  • immunocytochemistry
  • mesothelioma
  • platelet-derived growth factor

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology
  • Histology

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