TY - JOUR
T1 - Platelet-derived mitogenic activity and bone marrow fibrosis in myeloproliferative disorders
AU - Romano, Mario
AU - Viero, Piera
AU - Cortellazzo, Sergio
AU - Barbui, Tiziano
AU - Benedetta Donati, Maria
AU - Poggi, Andreina
PY - 1990
Y1 - 1990
N2 - Mitogenic activity, measured as 3H-thymidine incorporation by NIH 3T3 cells, following stimulation with platelet-rich-plasma-derived serum (PRS), platelet-poor-plasma-derived serum and platelet extract was studied in 14 patients with myeloproliferative disorders (MPD) and 7 normal subjects. Reduced mitogenic activity was found in PRS and platelet extract of patients with MPD, as compared to controls. The average levels of platelet-derived growth factor (PDGF) equivalents were as follows: 16.3 ± 7.2 pg/106 platelets in controls, 6.2 ± 2.2 pg/106 (p <0.05) platelets in patients with polycythaemia vera, 1.8 <0.4 pg/106 (p <0.01) platelets in patients with idiopathic myelofibrosis and 4.0 ± 0.8 pg/106 (p <0.05) platelets in patients with essential thrombocythaemia (Dunnett test). A reduction of intraplatelet levels of P-thromboglobulin, although not statistically significant, was found in the same patients. No apparent relation was found between the amount of PDGF equivalents and the degree of bone marrow fibrosis.
AB - Mitogenic activity, measured as 3H-thymidine incorporation by NIH 3T3 cells, following stimulation with platelet-rich-plasma-derived serum (PRS), platelet-poor-plasma-derived serum and platelet extract was studied in 14 patients with myeloproliferative disorders (MPD) and 7 normal subjects. Reduced mitogenic activity was found in PRS and platelet extract of patients with MPD, as compared to controls. The average levels of platelet-derived growth factor (PDGF) equivalents were as follows: 16.3 ± 7.2 pg/106 platelets in controls, 6.2 ± 2.2 pg/106 (p <0.05) platelets in patients with polycythaemia vera, 1.8 <0.4 pg/106 (p <0.01) platelets in patients with idiopathic myelofibrosis and 4.0 ± 0.8 pg/106 (p <0.05) platelets in patients with essential thrombocythaemia (Dunnett test). A reduction of intraplatelet levels of P-thromboglobulin, although not statistically significant, was found in the same patients. No apparent relation was found between the amount of PDGF equivalents and the degree of bone marrow fibrosis.
KW - Myelofibrosis
KW - Myeloproliferative disorders
KW - Platelet-derived growth factor
UR - http://www.scopus.com/inward/record.url?scp=0025299562&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025299562&partnerID=8YFLogxK
U2 - 10.1159/000216123
DO - 10.1159/000216123
M3 - Article
C2 - 2143744
AN - SCOPUS:0025299562
VL - 20
SP - 162
EP - 168
JO - Pathophysiology of Haemostasis and Thrombosis
JF - Pathophysiology of Haemostasis and Thrombosis
SN - 1424-8832
IS - 3
ER -