Platelet Lysate-Derived Neuropeptide y Influences Migration and Angiogenesis of Human Adipose Tissue-Derived Stromal Cells

Rita Businaro, Eleonora Scaccia, Antonella Bordin, Francesca Pagano, Mariangela Corsi, Camilla Siciliano, Raffaele Capoano, Eugenio Procaccini, Bruno Salvati, Vincenzo Petrozza, Pierangela Totta, Maria Teresa Vietri, Giacomo Frati, Elena De Falco

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Neuropeptide Y (NPY), a powerful neurotransmitter of the central nervous system, is a key regulator of angiogenesis and biology of adipose depots. Intriguingly, its peripheral vascular and angiogenic powerful activity is strictly associated to platelets, which are source of clinical hemoderivates, such as platelet lysate (PL), routinely employed in several clinical applications as wound healing, and to preserve ex vivo the progenitor properties of the adipose stromal cells pool. So far, the presence of NPY in PL and its biological effects on the adipose stromal cell fraction (ASCs) have never been investigated. Here, we aimed to identify endogenous sources of NPY such as PL-based preparations and to investigate which biological properties PL-derived NPY is able to exert on ASCs. The results show that PL contains a high amount of NPY, which is in part also excreted by ASCs when stimulated with PL. The protein levels of the three main NPY subtype receptors (Y1, Y2, Y5) are unaltered by stimulation of ASCs with PL, but their inhibition through selective pharmacological antagonists, considerably enhances migration, and a parallel reduction of angiogenic features of ASCs including decrease in VEGF mRNA and intracellular calcium levels, both downstream targets of NPY. The expression of VEGF and NPY is enhanced within the sites of neovascularisation of difficult wounds in patients after treatment with leuco-platelet concentrates. Our data highlight the presence of NPY in PL preparations and its peripheral effects on adipose progenitors.

Original languageEnglish
Article number14365
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - Dec 1 2018

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Stromal Cells
Neuropeptides
Neuropeptide Y
Adipose Tissue
Blood Platelets
Vascular Endothelial Growth Factor A
Wound Healing
Neurotransmitter Agents
Blood Vessels
Central Nervous System
Pharmacology
Calcium
Messenger RNA
Wounds and Injuries

ASJC Scopus subject areas

  • General

Cite this

Platelet Lysate-Derived Neuropeptide y Influences Migration and Angiogenesis of Human Adipose Tissue-Derived Stromal Cells. / Businaro, Rita; Scaccia, Eleonora; Bordin, Antonella; Pagano, Francesca; Corsi, Mariangela; Siciliano, Camilla; Capoano, Raffaele; Procaccini, Eugenio; Salvati, Bruno; Petrozza, Vincenzo; Totta, Pierangela; Vietri, Maria Teresa; Frati, Giacomo; De Falco, Elena.

In: Scientific Reports, Vol. 8, No. 1, 14365, 01.12.2018.

Research output: Contribution to journalArticle

Businaro, R, Scaccia, E, Bordin, A, Pagano, F, Corsi, M, Siciliano, C, Capoano, R, Procaccini, E, Salvati, B, Petrozza, V, Totta, P, Vietri, MT, Frati, G & De Falco, E 2018, 'Platelet Lysate-Derived Neuropeptide y Influences Migration and Angiogenesis of Human Adipose Tissue-Derived Stromal Cells', Scientific Reports, vol. 8, no. 1, 14365. https://doi.org/10.1038/s41598-018-32623-8
Businaro, Rita ; Scaccia, Eleonora ; Bordin, Antonella ; Pagano, Francesca ; Corsi, Mariangela ; Siciliano, Camilla ; Capoano, Raffaele ; Procaccini, Eugenio ; Salvati, Bruno ; Petrozza, Vincenzo ; Totta, Pierangela ; Vietri, Maria Teresa ; Frati, Giacomo ; De Falco, Elena. / Platelet Lysate-Derived Neuropeptide y Influences Migration and Angiogenesis of Human Adipose Tissue-Derived Stromal Cells. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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AU - Corsi, Mariangela

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