Platelet lysate embedded scaffolds for skin regeneration

Giuseppina Sandri, Maria Cristina Bonferoni, Silvia Rossi, Franca Ferrari, Michela Mori, Marila Cervio, Federica Riva, Ioannis Liakos, Athanassia Athanassiou, Francesca Saporito, Lara Marini, Carla Caramella

Research output: Contribution to journalArticlepeer-review

Abstract

Introduction: The work presents the development of acellular scaffolds extemporaneously embedded with platelet lysate (PL), as an innovative approach in the field of tissue regeneration/reparation. PL embedded scaffolds should have a tridimensional architecture to support cell migration and growth, in order to restore skin integrity. For this reason, chondroitin sulfate (CS) was associated with sodium alginate (SA) to prepare highly porous systems.Methods: The developed scaffolds were characterized for chemical stability to γ-radiation, morphology, hydration and mechanical properties. Moreover, the capability of fibroblasts and endothelial cells to populate the scaffold was evaluated by means of proliferation test 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and confocal laser scanning microscopy study. The scaffolds, not altered by sterilization, were characterized by limited swelling and high flexibility, by foam-like structure with bubbles that formed a high surface area and irregular texture suitable for cell adhesion.Results: Cell growth and scaffold population were evident on the bubble surface, where the cells appeared anchored to the scaffold structure.Conclusion: Scaffold network based on CS and SA demonstrated to be an effective support to enhance and to allow fibroblasts and endothelial cells (human umbilical vein endothelial cells, HUVEC) adhesion and proliferation. In particular, it could be hypothesized that cell adhesion was facilitated by the synergic effect of PL and CS. Although further in vivo evaluation is needed, on the basis of in vitro results, PL embedded scaffolds seem promising systems for skin wound healing.

Original languageEnglish
Pages (from-to)525-545
Number of pages21
JournalExpert Opinion on Drug Delivery
Volume12
Issue number4
DOIs
Publication statusPublished - Apr 1 2015

Keywords

  • Cell proliferation
  • Chondroitin sulfate
  • Platelet lysate
  • Sodium alginate
  • Tridimensional acellular scaffolds

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Medicine(all)

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