TY - JOUR
T1 - Platelet lysate embedded scaffolds for skin regeneration
AU - Sandri, Giuseppina
AU - Bonferoni, Maria Cristina
AU - Rossi, Silvia
AU - Ferrari, Franca
AU - Mori, Michela
AU - Cervio, Marila
AU - Riva, Federica
AU - Liakos, Ioannis
AU - Athanassiou, Athanassia
AU - Saporito, Francesca
AU - Marini, Lara
AU - Caramella, Carla
PY - 2015/4/1
Y1 - 2015/4/1
N2 - Introduction: The work presents the development of acellular scaffolds extemporaneously embedded with platelet lysate (PL), as an innovative approach in the field of tissue regeneration/reparation. PL embedded scaffolds should have a tridimensional architecture to support cell migration and growth, in order to restore skin integrity. For this reason, chondroitin sulfate (CS) was associated with sodium alginate (SA) to prepare highly porous systems.Methods: The developed scaffolds were characterized for chemical stability to γ-radiation, morphology, hydration and mechanical properties. Moreover, the capability of fibroblasts and endothelial cells to populate the scaffold was evaluated by means of proliferation test 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and confocal laser scanning microscopy study. The scaffolds, not altered by sterilization, were characterized by limited swelling and high flexibility, by foam-like structure with bubbles that formed a high surface area and irregular texture suitable for cell adhesion.Results: Cell growth and scaffold population were evident on the bubble surface, where the cells appeared anchored to the scaffold structure.Conclusion: Scaffold network based on CS and SA demonstrated to be an effective support to enhance and to allow fibroblasts and endothelial cells (human umbilical vein endothelial cells, HUVEC) adhesion and proliferation. In particular, it could be hypothesized that cell adhesion was facilitated by the synergic effect of PL and CS. Although further in vivo evaluation is needed, on the basis of in vitro results, PL embedded scaffolds seem promising systems for skin wound healing.
AB - Introduction: The work presents the development of acellular scaffolds extemporaneously embedded with platelet lysate (PL), as an innovative approach in the field of tissue regeneration/reparation. PL embedded scaffolds should have a tridimensional architecture to support cell migration and growth, in order to restore skin integrity. For this reason, chondroitin sulfate (CS) was associated with sodium alginate (SA) to prepare highly porous systems.Methods: The developed scaffolds were characterized for chemical stability to γ-radiation, morphology, hydration and mechanical properties. Moreover, the capability of fibroblasts and endothelial cells to populate the scaffold was evaluated by means of proliferation test 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and confocal laser scanning microscopy study. The scaffolds, not altered by sterilization, were characterized by limited swelling and high flexibility, by foam-like structure with bubbles that formed a high surface area and irregular texture suitable for cell adhesion.Results: Cell growth and scaffold population were evident on the bubble surface, where the cells appeared anchored to the scaffold structure.Conclusion: Scaffold network based on CS and SA demonstrated to be an effective support to enhance and to allow fibroblasts and endothelial cells (human umbilical vein endothelial cells, HUVEC) adhesion and proliferation. In particular, it could be hypothesized that cell adhesion was facilitated by the synergic effect of PL and CS. Although further in vivo evaluation is needed, on the basis of in vitro results, PL embedded scaffolds seem promising systems for skin wound healing.
KW - Cell proliferation
KW - Chondroitin sulfate
KW - Platelet lysate
KW - Sodium alginate
KW - Tridimensional acellular scaffolds
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UR - http://www.scopus.com/inward/citedby.url?scp=84925267238&partnerID=8YFLogxK
U2 - 10.1517/17425247.2015.961421
DO - 10.1517/17425247.2015.961421
M3 - Article
C2 - 25297510
AN - SCOPUS:84925267238
VL - 12
SP - 525
EP - 545
JO - Expert Opinion on Drug Delivery
JF - Expert Opinion on Drug Delivery
SN - 1742-5247
IS - 4
ER -