Platelet Lysate-Modified Porous Silicon Microparticles for Enhanced Cell Proliferation in Wound Healing Applications

Flavia Fontana, Michela Mori, Federica Riva, Ermei Mäkilä, Dongfei Liu, Jarno Salonen, Giovanni Nicoletti, Jouni Hirvonen, Carla Caramella, Hélder A. Santos

Research output: Contribution to journalArticlepeer-review


The new frontier in the treatment of chronic nonhealing wounds is the use of micro- and nanoparticles to deliver drugs or growth factors into the wound. Here, we used platelet lysate (PL), a hemoderivative of platelets, consisting of a multifactorial cocktail of growth factors, to modify porous silicon (PSi) microparticles and assessed both in vitro and ex vivo the properties of the developed microsystem. PL-modified PSi was assessed for its potential to induce proliferation of fibroblasts. The wound closure-promoting properties of the microsystem were then assessed in an in vitro wound healing assay. Finally, the PL-modified PSi microparticles were evaluated in an ex vivo experiment over human skin. It was shown that PL-modified PSi microparticles were cytocompatible and enhanced the cell proliferation in different experimental settings. In addition, this microsystem promoted the closure of the gap between the fibroblast cells in the wound healing assay, in periods of time comparable with the positive control, and induced a proliferation and regeneration process onto the human skin in an ex vivo experiment. Overall, our results show that PL-modified PSi microparticles are suitable microsystems for further development toward applications in the treatment of chronic nonhealing wounds.

Original languageEnglish
Pages (from-to)988-996
Number of pages9
JournalACS Applied Materials and Interfaces
Issue number1
Publication statusPublished - Jan 13 2016


  • fibroblast
  • microparticles
  • platelet lysate
  • porous silicon
  • wound healing

ASJC Scopus subject areas

  • Materials Science(all)


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