TY - JOUR
T1 - Platelet lysate promotes the expansion of T regulatory cells that favours in vitro wound healing by increasing keratinocyte migration and fibroblast production of extracellular matrix components
AU - Scopelliti, Fernanda
AU - Cattani, Caterina
AU - Dimartino, Valentina
AU - Scarponi, Claudia
AU - Madonna, Stefania
AU - Albanesi, Cristina
AU - Costanzo, Gianfranco
AU - Mirisola, Concetta
AU - Cavani, Andrea
N1 - Funding Information:
Acknowledgments: We thank Chiara Moncada and Gianluca Nicolella for the help in editing the final version of the manuscript, and Manuela Moncada for critical revision of the English language. Founding: This work was partially supported by the Italian Ministry of Health (grant number CUP J82F16000990005). Conflict of interests: none.
Publisher Copyright:
© 2020, JLE/Springer.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/1/1
Y1 - 2020/1/1
N2 - Background: Platelet lysate (PL) contains a cocktail of growth factors and cytokines that promote tissue repair and regeneration. In vitro studies have shown that PL may affect the reparative function of keratinocytes and fibroblasts, but little is known about the effect of PL on immune cells involved in wound healing. Objectives: To analyse the effects of PL on T cells involved in the wound repair process. Materials and Methods: The effect of PL on T cell proliferation, activation, and cytokine production was measured by ELISA and cytofluorometry and regulatory function based on cytofluorometry and Foxp3 RNA expression. Using an in vitro model of wound healing, we investigated the effect of PL-treated T cells on fibroblast proliferation and production of fibronectin and type-1 collagen as well as keratinocyte migration. Results: PL induced T lymphocyte proliferation and CD69 expression, and promoted a transient upregulation of IFN-γ and TNF-α. However, later on, PL enhanced the number of CD25+ T cells releasing TGF-β and expressing Foxp3 RNA, which was accompanied by a suppression in the level of type 1 cytokines. In the in vitro model, supernatants of PL-treated T cells positively affected the reparative capacity of human keratinocytes and induced fibroblast proliferation and production of fibronectin and type-1 collagen. Conclusion: These results indicate that PL temporally regulates T cells during the healing process, enhancing protective cytokines in the early phase, followed by a prominent expansion of TGF-β+ T regulatory cells that promote tissue regeneration and dampen the inflammatory response to prevent excessive tissue damage.
AB - Background: Platelet lysate (PL) contains a cocktail of growth factors and cytokines that promote tissue repair and regeneration. In vitro studies have shown that PL may affect the reparative function of keratinocytes and fibroblasts, but little is known about the effect of PL on immune cells involved in wound healing. Objectives: To analyse the effects of PL on T cells involved in the wound repair process. Materials and Methods: The effect of PL on T cell proliferation, activation, and cytokine production was measured by ELISA and cytofluorometry and regulatory function based on cytofluorometry and Foxp3 RNA expression. Using an in vitro model of wound healing, we investigated the effect of PL-treated T cells on fibroblast proliferation and production of fibronectin and type-1 collagen as well as keratinocyte migration. Results: PL induced T lymphocyte proliferation and CD69 expression, and promoted a transient upregulation of IFN-γ and TNF-α. However, later on, PL enhanced the number of CD25+ T cells releasing TGF-β and expressing Foxp3 RNA, which was accompanied by a suppression in the level of type 1 cytokines. In the in vitro model, supernatants of PL-treated T cells positively affected the reparative capacity of human keratinocytes and induced fibroblast proliferation and production of fibronectin and type-1 collagen. Conclusion: These results indicate that PL temporally regulates T cells during the healing process, enhancing protective cytokines in the early phase, followed by a prominent expansion of TGF-β+ T regulatory cells that promote tissue regeneration and dampen the inflammatory response to prevent excessive tissue damage.
KW - fibroblasts
KW - keratinocytes
KW - skin
KW - T lymphocytes
KW - T regulatory cells
KW - TGF-β
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U2 - 10.1684/ejd.2020.3711
DO - 10.1684/ejd.2020.3711
M3 - Article
C2 - 32250253
AN - SCOPUS:85083072163
VL - 30
SP - 3
EP - 11
JO - European Journal of Dermatology
JF - European Journal of Dermatology
SN - 1167-1122
IS - 1
ER -