Platelet mitochondrial dysfunction in critically ill patients

Comparison between sepsis and cardiogenic shock

Alessandro Protti, Francesco Fortunato, Andrea Artoni, Anna Lecchi, Giovanna Motta, Giovanni Mistraletti, Cristina Novembrino, Pietro P. Comi, Luciano Gattinoni

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Introduction: Platelet mitochondrial respiratory chain enzymes (that produce energy) are variably inhibited during human sepsis. Whether these changes occur even during other acute critical illness or are associated with impaired platelet aggregation and secretion (that consume energy) is not known. The aims of this study were firstly to compare platelet mitochondrial respiratory chain enzymes activity between patients with sepsis and those with cardiogenic shock, and secondly to study the relationship between platelet mitochondrial respiratory chain enzymes activity and platelet responsiveness to (exogenous) agonists in patients with sepsis. Methods: This was a prospective, observational, case-control study. Platelets were isolated from venous blood of 16 patients with severe sepsis or septic shock (free from antiplatelet drugs) and 16 others with cardiogenic shock, within 48 hours from admission to Intensive Care. Platelet mitochondrial respiratory chain enzymes activity was measured with spectrophotometry and expressed relative to citrate synthase activity, a marker of mitochondrial density. Platelet aggregation and secretion in response to adenosine di-phosphate (ADP), collagen, U46619 and thrombin receptor activating peptide were measured with lumiaggregometry only in patients with sepsis. In total, 16 healthy volunteers acted as controls for both spectrophotometry and lumiaggregometry. Results: Platelets of patients with sepsis or cardiogenic shock similarly had lower mitochondrial nicotinamide adenine dinucleotide dehydrogenase (NADH) (P2 0.36; P2 0.38; P2 0.27; P=0.002) and complex IV (R2 0.43; P

Original languageEnglish
Article number39
JournalCritical Care
Volume19
Issue number1
DOIs
Publication statusPublished - Feb 11 2015

Fingerprint

Cardiogenic Shock
Critical Illness
Sepsis
Blood Platelets
Electron Transport
thrombin receptor peptide SFLLRNP
Spectrophotometry
Enzymes
Platelet Aggregation
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
Citrate (si)-Synthase
Adenine Nucleotides
Platelet Aggregation Inhibitors
Critical Care
Septic Shock
NAD
Case-Control Studies
Healthy Volunteers
Oxidoreductases
Collagen

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

@article{05a16278a2ea408b80d30d3d596c5810,
title = "Platelet mitochondrial dysfunction in critically ill patients: Comparison between sepsis and cardiogenic shock",
abstract = "Introduction: Platelet mitochondrial respiratory chain enzymes (that produce energy) are variably inhibited during human sepsis. Whether these changes occur even during other acute critical illness or are associated with impaired platelet aggregation and secretion (that consume energy) is not known. The aims of this study were firstly to compare platelet mitochondrial respiratory chain enzymes activity between patients with sepsis and those with cardiogenic shock, and secondly to study the relationship between platelet mitochondrial respiratory chain enzymes activity and platelet responsiveness to (exogenous) agonists in patients with sepsis. Methods: This was a prospective, observational, case-control study. Platelets were isolated from venous blood of 16 patients with severe sepsis or septic shock (free from antiplatelet drugs) and 16 others with cardiogenic shock, within 48 hours from admission to Intensive Care. Platelet mitochondrial respiratory chain enzymes activity was measured with spectrophotometry and expressed relative to citrate synthase activity, a marker of mitochondrial density. Platelet aggregation and secretion in response to adenosine di-phosphate (ADP), collagen, U46619 and thrombin receptor activating peptide were measured with lumiaggregometry only in patients with sepsis. In total, 16 healthy volunteers acted as controls for both spectrophotometry and lumiaggregometry. Results: Platelets of patients with sepsis or cardiogenic shock similarly had lower mitochondrial nicotinamide adenine dinucleotide dehydrogenase (NADH) (P2 0.36; P2 0.38; P2 0.27; P=0.002) and complex IV (R2 0.43; P",
author = "Alessandro Protti and Francesco Fortunato and Andrea Artoni and Anna Lecchi and Giovanna Motta and Giovanni Mistraletti and Cristina Novembrino and Comi, {Pietro P.} and Luciano Gattinoni",
year = "2015",
month = "2",
day = "11",
doi = "10.1186/s13054-015-0762-7",
language = "English",
volume = "19",
journal = "Critical Care",
issn = "1466-609X",
publisher = "Springer Science + Business Media",
number = "1",

}

TY - JOUR

T1 - Platelet mitochondrial dysfunction in critically ill patients

T2 - Comparison between sepsis and cardiogenic shock

AU - Protti, Alessandro

AU - Fortunato, Francesco

AU - Artoni, Andrea

AU - Lecchi, Anna

AU - Motta, Giovanna

AU - Mistraletti, Giovanni

AU - Novembrino, Cristina

AU - Comi, Pietro P.

AU - Gattinoni, Luciano

PY - 2015/2/11

Y1 - 2015/2/11

N2 - Introduction: Platelet mitochondrial respiratory chain enzymes (that produce energy) are variably inhibited during human sepsis. Whether these changes occur even during other acute critical illness or are associated with impaired platelet aggregation and secretion (that consume energy) is not known. The aims of this study were firstly to compare platelet mitochondrial respiratory chain enzymes activity between patients with sepsis and those with cardiogenic shock, and secondly to study the relationship between platelet mitochondrial respiratory chain enzymes activity and platelet responsiveness to (exogenous) agonists in patients with sepsis. Methods: This was a prospective, observational, case-control study. Platelets were isolated from venous blood of 16 patients with severe sepsis or septic shock (free from antiplatelet drugs) and 16 others with cardiogenic shock, within 48 hours from admission to Intensive Care. Platelet mitochondrial respiratory chain enzymes activity was measured with spectrophotometry and expressed relative to citrate synthase activity, a marker of mitochondrial density. Platelet aggregation and secretion in response to adenosine di-phosphate (ADP), collagen, U46619 and thrombin receptor activating peptide were measured with lumiaggregometry only in patients with sepsis. In total, 16 healthy volunteers acted as controls for both spectrophotometry and lumiaggregometry. Results: Platelets of patients with sepsis or cardiogenic shock similarly had lower mitochondrial nicotinamide adenine dinucleotide dehydrogenase (NADH) (P2 0.36; P2 0.38; P2 0.27; P=0.002) and complex IV (R2 0.43; P

AB - Introduction: Platelet mitochondrial respiratory chain enzymes (that produce energy) are variably inhibited during human sepsis. Whether these changes occur even during other acute critical illness or are associated with impaired platelet aggregation and secretion (that consume energy) is not known. The aims of this study were firstly to compare platelet mitochondrial respiratory chain enzymes activity between patients with sepsis and those with cardiogenic shock, and secondly to study the relationship between platelet mitochondrial respiratory chain enzymes activity and platelet responsiveness to (exogenous) agonists in patients with sepsis. Methods: This was a prospective, observational, case-control study. Platelets were isolated from venous blood of 16 patients with severe sepsis or septic shock (free from antiplatelet drugs) and 16 others with cardiogenic shock, within 48 hours from admission to Intensive Care. Platelet mitochondrial respiratory chain enzymes activity was measured with spectrophotometry and expressed relative to citrate synthase activity, a marker of mitochondrial density. Platelet aggregation and secretion in response to adenosine di-phosphate (ADP), collagen, U46619 and thrombin receptor activating peptide were measured with lumiaggregometry only in patients with sepsis. In total, 16 healthy volunteers acted as controls for both spectrophotometry and lumiaggregometry. Results: Platelets of patients with sepsis or cardiogenic shock similarly had lower mitochondrial nicotinamide adenine dinucleotide dehydrogenase (NADH) (P2 0.36; P2 0.38; P2 0.27; P=0.002) and complex IV (R2 0.43; P

UR - http://www.scopus.com/inward/record.url?scp=84924272613&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84924272613&partnerID=8YFLogxK

U2 - 10.1186/s13054-015-0762-7

DO - 10.1186/s13054-015-0762-7

M3 - Article

VL - 19

JO - Critical Care

JF - Critical Care

SN - 1466-609X

IS - 1

M1 - 39

ER -